[en] Respiratory diseases, including inflammatory airway disease (IAD), viral and bacterial infections, are common problems in exercising horses. The airway epithelium constitutes a major physical barrier against airborne infections and plays an essential role in the lung innate immune response mainly through toll-like receptor (TLR) activation. The aim of this study was to develop a model for the culture of equine bronchial epithelial cells (EBEC) in vitro and to explore EBEC innate immune responses in trained horses. Bronchial epithelial biopsies were taken from 6 adult horses during lower airway endoscopy. EBEC were grown in vitro by an explant method. The innate immune response of EBEC was evaluated in vitro by treatment with TLR ligands. TLR3 is the most strongly expressed TLR at the mRNA level in EBEC and stimulation of EBEC with Poly(I:C), an analog of viral dsRNA, triggers a strong secretion of IFN-β, TNF-α, IL-6 and CXCL8. We further evaluated the EBEC innate immune response in horses that underwent a 4-month-training program. While training had no effect on TLR mRNA expression in EBEC as well as in bronchial biopsies, it increased the production of IFN-β after stimulation with a TLR3 ligand and decreased the secretion of TNF-α and IL-6 after stimulation with a TLR2 and TLR3 ligand. These findings may be implicated in the increased risk for viral and bacterial infections observed in sport horses. Altogether, we report a successful model for the culture of EBEC that can be applied to the investigation of pathophysiologic conditions in longitudinal studies.
Research center :
FARAH - Fundamental and Applied Research for Animals and Health - ULiège
Disciplines :
Veterinary medicine & animal health Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Frellstedt, Linda ; Université de Liège - ULiège > Département de sciences fonctionnelles > Phys. neuro-muscul., de l'effort - Méd. sport. des animaux
Gosset, Philippe; Institut Pasteur (Lille) > Institut Pasteur de Lille, Centre d’Infection et d’Immunité de Lille, Lille, France
Kervoaze, Gwenola; Institut Pasteur (Lille) > Institut Pasteur de Lille, Centre d’Infection et d’Immunité de Lille, Lille, France
Hans, Aymeric; Anses – Dozulé, Equine Pathology Laboratory, Virology Unit, Goustranville, France
Desmet, Christophe ; Université de Liège - ULiège > Département de sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire
Pirottin, Dimitri ; Université de Liège - ULiège > Département de sciences fonctionnelles (DSF) > Département de sciences fonctionnelles (DSF)
Bureau, Fabrice ; Université de Liège - ULiège > Département de sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire
Lekeux, Pierre ; Université de Liège - ULiège > Département de sciences fonctionnelles (DSF) > Physiologie
Art, Tatiana ; Université de Liège - ULiège > Département de sciences fonctionnelles (DSF) > Phys. neuro-muscul., de l'effort - Méd. sport. des animaux
Language :
English
Title :
The Innate Immune Response of Equine Bronchial Epithelial Cells is Altered by Training
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