[en] VP22, encoded by theUL49gene of Marek’s disease virus (MDV), is indispensable for virus cell-to-cell spreading. We show herein that MDVUL49can be functionally replaced with avian and human viral orthologs. Replacement of MDV VP22 with that of avian gallid herpesvirus 3 or herpesvirus of turkey, whose residue identity with MDV is close to 60%, resulted in 73 and 131% changes in viral spreading, respectively. In contrast, VP22 replacement with human herpes simplex virus type 1 resulted in 14% plaque formation. Therefore, heterologous avian and human VP22 proteins share sufficient structural homology to support MDV cell-to-cell spreading, albeit with different efficiencies.
Disciplines :
Life sciences: Multidisciplinary, general & others
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