Purification and identification of novel antioxidant peptides from enzymatic hydrolysate of chickpea (Cicer arietinum L.) protein concentrate

Ghribi, Abir Mokni; Sila, Assaâd; Przybylski, Rémi; Nedjar-Arroume, Naima; Makhlouf, Ines; Blecker, Christophe; Attia, Hamadi; Dhulster, Pascal; Bougatef, Ali; Besbes, Souhail

doi:10.1016/j.jff.2014.12.011

Article (Scientific journals)

Purification and identification of novel antioxidant peptides from enzymatic hydrolysate of chickpea (Cicer arietinum L.) protein concentrate

Ghribi, Abir Mokni; Sila, Assaâd; Przybylski, Rémi et al.

2015 • In Journal of Functional Foods, 12, p. 516-525

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/178526

DOI
10.1016/j.jff.2014.12.011

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Keywords :

Chickpea, Protein concentrate; Antioxidant activity, Peptide; Mass spectrometry

Abstract :

[en] Enzymatic hydrolysis of chickpea protein concentrate (CP) by Alcalase® and some physiochemical and antioxidant properties of the resulting hydrolysate (CPH) were characterised. CPH displayed higher antioxidant activity than CP. This hydrolysate was fractionated by size exclusion chromatography on a Sephadex G-25 into four major fractions (Fra.I, Fra.II, Fra.III, and Fra.IV). Fraction III, which exhibited the highest DPPH scavenging activity (54% at 1 mg/ml), was then fractionated by reversed-phase high performance liquid chromatography (RP-HPLC). Eleven antioxidant fractions were isolated and two peptide subfractions show antioxidant activity (P3 and P8). The P8 displayed the highest DPPH radicalscavenging activity (67%; at 200 g/ml) among these peptides subfractions. The molecular masses and amino acids sequences of the purified peptides were determined using ESIMS and ESIMS/MS, respectively. Their structures were identified as Asp-His-Gly and Val- Gly-Asp-Ile. These peptides did not show haemolytic activity towards bovine erythrocytes. The results suggest that CPH are good source of natural antioxidants.

Disciplines :

Food science

Author, co-author :

Ghribi, Abir Mokni

Sila, Assaâd

Przybylski, Rémi

Nedjar-Arroume, Naima

Makhlouf, Ines

Blecker, Christophe ; Université de Liège - ULiège > Chimie et bio-industries > Science des alim. et formul.

Attia, Hamadi

Dhulster, Pascal

Bougatef, Ali

Besbes, Souhail

Language :

English

Title :

Purification and identification of novel antioxidant peptides from enzymatic hydrolysate of chickpea (Cicer arietinum L.) protein concentrate

Publication date :

January 2015

Journal title :

Journal of Functional Foods

ISSN :

1756-4646

eISSN :

2214-9414

Publisher :

Elsevier, Netherlands

Volume :

Pages :

516-525

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 21 February 2015

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