[en] The effect of acid and osmotic shifts on the growth of Listeria monocytogenes was evaluated at 10 degrees C. Two types of shifts were tested: (i) within the range of pH and water activity (a(w)) levels that allow growth of L. monocytogenes and (ii) after habituation at no-growth conditions back to growth-permitting conditions. A L. monocytogenes cheese isolate, with high survival capacity during cheesemaking, was inoculated (10(2) CFU/ml) in tryptic soy broth supplemented with 0.6% yeast extract at six pH levels (5.1 to 7.2; adjusted with lactic acid) and 0.5% NaCl (a(w) 0.995), or four a(w) levels (0.995 to 0.93, adjusted with 0.5 to 10.5% NaCl) at pH 7.2 and grown to early stationary phase. L. monocytogenes was then shifted (at 10(2) CFU/ml) to each of the aforementioned growth-permitting pH and a(w) levels and incubated at 10 degrees C. Shifts from no-growth to growth-permitting conditions were carried out by transferring L. monocytogenes habituated at pH 4.9 or a(w) 0.90 (12.5% NaCl) for 1, 5, and 10 days to all pH and a(w) levels permitting growth. Reducing a(w) or pH at different levels in the range of 0.995 to 0.93 and 7.2 to 5.1, respectively, decreased the maximum specific growth rate of L. monocytogenes. The lag time of the organism increased with all osmotic downshifts, as well as by the reduction of pH to 5.1. Conversely, any type of shift within pH 5.5 to 7.2 did not markedly affect the lag times of L. monocytogenes. The longer the cells were incubated at no-growth a(w) (0.90), the faster they initiated growth subsequently, suggesting adaptation to osmotic stress. Conversely, extended habituation at pH 4.9 had the opposite effect on subsequent growth of L. monocytogenes, possibly due to cell injury. These results suggest that there is an adaptation or injury rate induced at conditions inhibiting the growth of the pathogen. Thus, quantifying adaptation phenomena under growth-limiting environments, such as in fermented dairy and meat products or products preserved in brine, is essential for reliable growth simulations of L monocytogenes during transportation and storage of foods.
Disciplines :
Food science
Author, co-author :
Belessi, C.-I. A.
Le Marc, Y.
Merkouri, S. I.
Gounadaki, A. S.
Schvartzman Echenique, Maria Sol ; Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Jordan, K.
Drosinos, E. H.
Skandamis, P. N.
Language :
English
Title :
Adaptive growth responses of Listeria monocytogenes to acid and osmotic shifts above and across the growth boundaries.
Publication date :
2011
Journal title :
Journal of Food Protection
ISSN :
0362-028X
eISSN :
1944-9097
Publisher :
International Association for Food Protection, United States - Iowa
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Bibliography
Alavi, S. H., V. M. Puri, S. J. Knabel, R. H. Mohtar, and R. H. Whiting. 1999. Development and validation of a dynamic growth model for Listeria monocytogenes in fluid whole milk. J. Food Prot. 62:170-176.
Augustin, J. C., L. Rosso, and V. Carlier. 2000. A model describing the effect of temperature history on lag time for Listeria monocytogenes. Int. J. Food Microbiol. 57:169-181.
Baranyi, J., and T. A. Roberts. 1994. A dynamic approach to predicting bacterial growth in food. Int. J. Food Microbiol. 23:277-294.
Baranyi, J., T. P. Robinson, A. Kaloti, and B. M. Mackey. 1995. Predicting growth of Brochothrix thermosphacta at changing temperature. Int. J. Food Microbiol. 27:61-75.
Cheroutre-Vialette, M., and A. Lebert. 2000. Modelling the growth of Listeria monocytogenes in dynamic conditions. Int. J. Food Microbiol. 55:201-207.
Davis, M. J., P. J. Coote, and P. O'Byrne. 1996. Acid tolerance in Listeria monocytogenes: the adaptive acid tolerance response (ATR) and growth-phase-dependent acid resistance. Microbiology 142:2975-2982.
Delignette-Muller, M. L., F. Baty, M. Cornu, and H. Bergis. 2005. Modelling the effect of a temperature shift on the lag phase duration of Listeria monocytogenes. Int. J. Food Microbiol. 100:77-84.
Gay, M., O. Cerf, and K. R. Davey. 1996. Significance of preincubation temperature and inoculum concentration on subsequent growth of Listeria monocytogenes at 14°C. J. Appl. Microbiol. 81:433-438.
Goodson, M., and R. J. Rowbury. 1989. Habituation to normally lethal acidity by prior growth of Escherichia coli at a sub-lethal acid pH value. Lett. Appl. Microbiol. 8:77-79.
Hill, C., P. D. Cotter, R. D. Sleator, and C. G. M. Gahan. 2002. Bacterial stress response in Listeria monocytogenes: jumping the hurdles imposed by minimal processing. Int. Dairy J. 12:273-283.
Ko, R., L. T. Smith, and G. M. Smith. 1994. Glycine betaine confers enhanced osmotolerance and cryotolerance on Listeria monocytogenes. J. Bacteriol. 176:426-431.
Kroll, R. G., and R. A. Patchett. 1992. Induced acid tolerance in Listeria monocytogenes. Lett. Appl. Microbiol. 14:224-227.
Lebert, I., C. G. Dussap, and A. Lebert. 2004. Effect of aw, controlled by the addition of solutes or by water content, on the growth of Listeria innocua in broth and in a gelatine model. Int. J. Food Microbiol. 94:67-78.
Lin, J., M. P. Smith, K. C. Chapin, H. B. Baik, G. N. Bennet, and J. W. Foster. 1996. Mechanisms of acid resistance in Enterohemorrhagic Escherichia coli. Appl. Environ. Microbiol. 62:3094-3100.
Mellefont, L. A., T. A. McMeekin, and T. Ross. 2003. The effect of abrupt osmotic shifts on the lag phase duration of foodborne bacteria. Int. J. Food Microbiol. 83:281-293.
Mellefont, L. A., T. A. McMeekin, and T. Ross. 2004. The effect of abrupt osmotic shifts on the lag phase duration of physiologically distinct populations of Salmonella typhimurium. Int. J. Food Microbiol. 99:111-120.
Mellefont, L. A., T. A. McMeekin, and T. Ross. 2005. Viable count estimates of lag time responses for Salmonella typhimurium M48 subjected to abrupt osmotic shifts. Int. J. Food Microbiol. 105:399-410.
Mellefont, L. A., and T. Ross. 2003. The effect of abrupt shifts in temperature on the lag phase duration of Escherichia coli and Klebsiella oxytoca. Int. J. Food Microbiol. 83:295-305.
Membré, J.-M., T. Ross, and T. A. McMeekin. 1998. Behaviour of Listeria monocytogenes under combined chilling processes. Lett. Appl. Microbiol. 28:216-220.
Mitchell, G. A., T. F. Brocklehurst, R. Parker, and A. C. Smith. 1994. The effect of transient temperatures on the growth of Salmonella typhimurium LT2. I: cycling within the growth region. J. Appl. Microbiol. 77:113-119.
Mitchell, G. A., T. F. Brocklehurst, R. Parker, and A. C. Smith. 1995. The effect of transient temperatures on the growth of Salmonella typhimurium LT2. II: excursions outside the growth region. J. Appl. Microbiol. 79:128-134.
Robinson, T. P., O. O. Aboaba, A. Kaloti, M. J. Ocio, J. Baranyi, and B. N. Mackey. 2001. The effect of inoculum size on the lag phase of Listeria monocytogenes. Int. J. Food Microbiol. 70:163-173.
Robinson, T. P., M. J. Ocio, A. Kaloti, and B. M. Mackey. 1998. The effect of the growth environment on the lag phase of Listeria monocytogenes. Int. J. Food Microbiol. 44:83-92.
Shabala, L., S. H. Lee, P. Cannesson, and T. Ross. 2008. Acid and NaCl limits of growth of Listeria monocytogenes and influence of sequence of inimical acid and NaCl levels on inactivation kinetics. J. Food Prot. 71:1169-1177.
Skandamis, P. N., J. D. Stopforth, P. A. Kendall, K. E. Belk, J. A. Scanga, G. C. Smith, and J. N. Sofos. 2007. Modeling the effect of inoculum size and acid adaptation on growth/no growth interface of Escherichia coli O157:H7. Int. J. Food Microbiol. 120:237-249.
Skandamis, P. N., Y. Yoon, J. D. Stopforth, P. A. Kendall, and J. N. Sofos. 2008. Heat and acid tolerance of Listeria monocytogenes after exposure to single and multiple sublethal stresses. Food Microbiol. 25:294-303.
Swinnen, I. A. M., K. Bernaerts, K. Gysemans, and J. F. Van Impe. 2005. Quantifying microbial lag phenomena due to a sudden rise in temperature: a systematic macroscopic study. Int. J. Food Microbiol. 100:85-96.
Swinnen, I. A. M., K. Bernaerts, and J. F. Van Impe. 2006. Modelling the work to be done by Escherichia coli to adapt to sudden temperature upshifts. Lett. Appl. Microbiol. 42:507-513.
Tiganitas, T., N. Zeaki, A. S. Gounadaki, E. H. Drosinos, and P. N. Skandamis. 2009. Study of the effect of lethal and sublethal pH and aw stresses on the inactivation or growth of Listeria monocytogenes and Salmonella Typhimurium. Int. J. Food Microbiol. 134:104-112.
Whiting, R. C., and L. G. Bagi. 2002. Modelling the lag phase of Listeria monocytogenes. Int. J. Food Microbiol. 73:291-295.
Zwietering, M. H., J. C. De Wit, H. G. A. M. Cuppers, and K. Van't Riet. 1994. Modeling of bacterial growth with shifts in temperature. Appl. Environ. Microbiol. 60:204-213.
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