2014 • In Journal of Crustacean Biology: A Quarterly of the Crustacean Society for the Publication of Research on Any Aspect of the Biology of Crustacea, 34 (5), p. 539-551
[en] On the basis of macroscopic aspects (body and eye colour, lipid droplets), it was possible to sort specimens of Gammarus pulex (Linnaeus, 1758) into five categories that correspond to moult periods and stages (A, B, C, D1 and D2) based on integument features (tergite cuticle stiffness, layers and thickness). These stages also correspond to changes in digestive tract histology (gut content, cell ultrastructure, and lipid storage). With reference to the pereion tergite integument, this makes it possible to standardize moulting stage terminology and criteria with those applied to decapods while validating a quick, simple, moult-staging method that avoids injury and informs us about the physiology of the whole organism. The moult cycle was very short (about 12-15 days), with a "virtual," practically non-existent, anecdysis or "integument resting period" between post-ecdysis and pre-ecdysis. The pore canals previously known to be "open to the outside" appeared closed at early post-ecdysis by a lipid-rich fillng material that could be responsible for the cuticular waterproofing barrier allowing mineral deposition. In the digestive tract, the main structural changes were late post-ecdysial loss of midgut cells and digestive gland B-cells (probably by extrusion) when restarting the feeding cycle. Pre-ecdysial increase and post-ecdysial decrease in storage lipids are also obvious. We present a quick moult-staging method to sort a great number of G. pulex for physiological or toxicological assays investigating how animals at specific periods of their moult cycle respond to both natural and anthropogenic environmental changes.
Trevisan, Mélissa ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Laboratoire de Morphologie fonctionnelle et évolutive
Leroy, Delphine; Université de Liège - ULiège > Biologie, Ecologie & Evolution > Laboratoire d'Ecologie Animale et d'Ecotoxicologie
Decloux, Nicole ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Laboratoire de Morphologie Fonctionnelle et Evolutive
Thomé, Jean-Pierre ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie
Compère, Philippe ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Laboratoire de Morphologie Fonctionnelle et Evolutive
Titre :
Moult-related changes in the integument, midgut, and digestive gland in the freshwater amphipod gammarus pulex
Date de publication/diffusion :
10 septembre 2014
Titre du périodique :
Journal of Crustacean Biology: A Quarterly of the Crustacean Society for the Publication of Research on Any Aspect of the Biology of Crustacea
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