Article (Scientific journals)
Approaches for analyzing the roles of mast cells and their proteases in vivo.
Galli, Stephen J.; Tsai, Mindy; Marichal, Thomas et al.
2015In Advances in Immunology, 126, p. 45-127
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Keywords :
Animals; Disease Models, Animal; Humans; Immunoglobulin E/immunology; Mast Cells/enzymology/immunology; Mice; Peptide Hydrolases/classification/physiology; Basophils; Cre recombinase; Mouse model; Stem cell factor; c-kit
Abstract :
[en] The roles of mast cells in health and disease remain incompletely understood. While the evidence that mast cells are critical effector cells in IgE-dependent anaphylaxis and other acute IgE-mediated allergic reactions seems unassailable, studies employing various mice deficient in mast cells or mast cell-associated proteases have yielded divergent conclusions about the roles of mast cells or their proteases in certain other immunological responses. Such "controversial" results call into question the relative utility of various older versus newer approaches to ascertain the roles of mast cells and mast cell proteases in vivo. This review discusses how both older and more recent mouse models have been used to investigate the functions of mast cells and their proteases in health and disease. We particularly focus on settings in which divergent conclusions about the importance of mast cells and their proteases have been supported by studies that employed different models of mast cell or mast cell protease deficiency. We think that two major conclusions can be drawn from such findings: (1) no matter which models of mast cell or mast cell protease deficiency one employs, the conclusions drawn from the experiments always should take into account the potential limitations of the models (particularly abnormalities affecting cell types other than mast cells) and (2) even when analyzing a biological response using a single model of mast cell or mast cell protease deficiency, details of experimental design are critical in efforts to define those conditions under which important contributions of mast cells or their proteases can be identified.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Galli, Stephen J.
Tsai, Mindy
Marichal, Thomas  ;  Université de Liège - ULiège > Département de sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire
Tchougounova, Elena
Reber, Laurent L.
Pejler, Gunnar
Language :
English
Title :
Approaches for analyzing the roles of mast cells and their proteases in vivo.
Publication date :
2015
Journal title :
Advances in Immunology
ISSN :
0065-2776
eISSN :
1557-8445
Publisher :
Elsevier, United States
Volume :
126
Pages :
45-127
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
(c) 2015 Elsevier Inc. All rights reserved.
Available on ORBi :
since 23 December 2014

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