Exploiting the Burkholderia pseudomallei acute phase antigen BPSL2765 for structure-based epitope discovery/design in structural vaccinology.

Antibodies/blood/immunology; Antigens, Bacterial/chemistry/immunology/metabolism; Bacterial Proteins/chemistry/genetics/immunology; Bacterial Vaccines/immunology; Burkholderia pseudomallei/immunology/metabolism; Crystallography, X-Ray; Epitope Mapping; Epitopes/chemistry/immunology/metabolism; Humans; Molecular Dynamics Simulation; Neutrophils/cytology/immunology; Phagocytosis; Protein Structure, Tertiary; Recombinant Proteins/biosynthesis/chemistry/immunology

Abstract :

[en] We solved the crystal structure of Burkholderia pseudomallei acute phase antigen BPSL2765 in the context of a structural vaccinology study, in the area of melioidosis vaccine development. Based on the structure, we applied a recently developed method for epitope design that combines computational epitope predictions with in vitro mapping experiments and successfully identified a consensus sequence within the antigen that, when engineered as a synthetic peptide, was selectively immunorecognized to the same extent as the recombinant protein in sera from melioidosis-affected subjects. Antibodies raised against the consensus peptide were successfully tested in opsonization bacterial killing experiments and antibody-dependent agglutination tests of B. pseudomallei. Our strategy represents a step in the development of immunodiagnostics, in the production of specific antibodies and in the optimization of antigens for vaccine development, starting from structural and physicochemical principles.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Gourlay, Louise J.

Peri, Claudio

Ferrer-Navarro, Mario

Conchillo-Sole, Oscar

Gori, Alessandro

Rinchai, Darawan

Thomas, Rachael J.

Champion, Olivia L.

Michell, Stephen L.

Kewcharoenwong, Chidchamai

More authors (9 more)

Language :

English

Title :

Exploiting the Burkholderia pseudomallei acute phase antigen BPSL2765 for structure-based epitope discovery/design in structural vaccinology.

Publication date :

2013

Journal title :

Chemistry and Biology

ISSN :

1074-5521

eISSN :

1879-1301

Publisher :

Cell Press, Cambridge, United States - Massachusetts

Volume :

Issue :

Pages :

1147-56

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBi :

since 22 December 2014

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