Article (Scientific journals)
Redefining the PF06864 Pfam family based on Burkholderia pseudomallei PilO2(Bp) S-SAD crystal structure.
Lassaux, Patricia; Conchillo-Sole, Oscar; Manjasetty, Babu A. et al.
2014In PLoS ONE, 9 (4), p. 94981
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Abstract :
[en] Type IV pili are surface-exposed filaments and bacterial virulence factors, represented by the Tfpa and Tfpb types, which assemble via specific machineries. The Tfpb group is further divided into seven variants, linked to heterogeneity in the assembly machineries. Here we focus on PilO2(Bp), a protein component of the Tfpb R64 thin pilus variant assembly machinery from the pathogen Burkholderia pseudomallei. PilO2(Bp) belongs to the PF06864 Pfam family, for which an improved definition is presented based on newly derived Hidden Markov Model (HMM) profiles. The 3D structure of the N-terminal domain of PilO2(Bp) (N-PilO2(Bp)), here reported, is the first structural representative of the PF06864 family. N-PilO2(Bp) presents an actin-like ATPase fold that is shown to be present in BfpC, a different variant assembly protein; the new HMM profiles classify BfpC as a PF06864 member. Our results provide structural insight into the PF06864 family and on the Type IV pili assembly machinery.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Lassaux, Patricia ;  Université de Liège - ULiège > GIGA-R : Virologie - Immunologie
Conchillo-Sole, Oscar
Manjasetty, Babu A.
Yero, Daniel
Perletti, Lucia
Belrhali, Hassan
Daura, Xavier
Gourlay, Louise J.
Bolognesi, Martino
Language :
English
Title :
Redefining the PF06864 Pfam family based on Burkholderia pseudomallei PilO2(Bp) S-SAD crystal structure.
Publication date :
2014
Journal title :
PLoS ONE
eISSN :
1932-6203
Publisher :
Public Library of Science, United States - California
Volume :
9
Issue :
4
Pages :
e94981
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 December 2014

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