DiC14-amidine cationic liposomes stimulate myeloid dendritic cells through Toll-like receptor 4.

Adaptor Proteins, Vesicular Transport/genetics; Adjuvants, Immunologic/administration & dosage/pharmacology; Amidines/administration & dosage/pharmacology; Animals; Cell Line; Cytokines/metabolism; Dendritic Cells/drug effects/metabolism; Female; Humans; Interleukin-12 Subunit p40/metabolism; Lymphocyte Antigen 96/genetics; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Knockout; Mice, Mutant Strains; Mitogen-Activated Protein Kinases/metabolism; Myeloid Cells/cytology; Myeloid Differentiation Factor 88/genetics; NF-kappa B/metabolism; Phosphorylation/drug effects; Toll-Like Receptor 4/agonists/genetics; Toll-Like Receptors/genetics; Transfection; Tumor Necrosis Factor-alpha/metabolism

Abstract :

[en] DiC14-amidine cationic liposomes were recently shown to promote Th1 responses when mixed with allergen. To further define the mode of action of diC14-amidine as potential vaccine adjuvant, we characterized its effects on mouse and human myeloid dendritic cells (DC). First, we observed that, as compared with two other cationic liposomes, only diC14-amidine liposomes induced the production of IL-12p40 and TNF-alpha by mouse bone marrow-derived DC. DiC14-amidine liposomes also activated human DC, as shown by synthesis of IL-12p40 and TNF-alpha, accumulation of IL-6, IFN-beta and CXCL10 mRNA, and up-regulation of membrane expression of CD80 and CD86. DC stimulation by diC14-amidine liposomes was associated with activation of NF-kappaB, ERK1/2, JNK and p38 MAP kinases. Finally, we demonstrated in mouse and human cells that diC14-amidine liposomes use Toll-like receptor 4 to elicit both MyD88-dependent and Toll/IL-1R-containing adaptor inducing interferon IFN-beta (TRIF)-dependent responses.

Disciplines :

Genetics & genetic processes

Author, co-author :

Tanaka, Tetsuya

Legat, Amandine

Adam, Emmanuelle

Steuve, Jonathan

GATOT, Jean-Stéphane ; Centre Hospitalier Universitaire de Liège - CHU > Génétique

Vandenbranden, Michel

Ulianov, Liliana

Lonez, Caroline

Ruysschaert, Jean-Marie

Muraille, Eric

More authors (3 more)

Language :

English

Title :

DiC14-amidine cationic liposomes stimulate myeloid dendritic cells through Toll-like receptor 4.

Publication date :

2008

Journal title :

European Journal of Immunology

ISSN :

0014-2980

eISSN :

1521-4141

Publisher :

John Wiley & Sons, United Kingdom

Volume :

Issue :

Pages :

1351-7

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 26 November 2014

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Bibliography

Elouahabi, A. and Ruysschaert, J. M., Formation and intracellular trafficking of lipoplexes and polyplexes. Mol. Ther. 2005. 11: 336-347.
Bei, R., Guptill V., Masuelli, L., Kashmiri, S. V., Muraro, R., Frati, L., Schlom, J. and Kantor, J., The use of a cationic liposome formulation (DOTAP) mixed with a recombinant tumor-associated antigen to induce immune responses and protective immunity in mice. J. Immunother. 1998. 21: 159-169.
Brunel, F., Darbouret, A. and Ronco, J., Cationic lipid DC-Chol induces an improved and balanced immunity able to overcome the unresponsiveness to the hepatitis B vaccine. Vaccine 1999. 17: 2192-2203.
Hartikka, J., Bozoukova, V., Ferrari, M., Sukhu, L., Enas, J., Sawdey, M., Wloch, M. K. et al., Vaxfectin enhances the humoral immune response to plasmid DNA-encoded antigens. Vaccine 2001. 19: 1911-1923.
Jiao, X., Wang, R. Y., Feng, Z., Alter, H. J. and Shih, J. W., Modulation of cellular immune response against hepatitis C virus nonstructural protein 3 by cationic liposome encapsulated DNA immunization. Hepatology 2003. 37: 452-460.
Mitchell, L. A., Joseph, A., Kedar, E., Barenholz, Y. and Galun, E., Mucosal immunization against hepatitis A: Antibody responses are enhanced by co-administration of synthetic oligodeoxynucleotides and a novel cationic lipid. Vaccine 2006. 19: 5300-5310.
Naito, T., Kaneko, Y. and Kozbor, D., Oral vaccination with modified vaccinia virus Ankara attached covalently to TMPEG-modified cationic liposomes overcomes pre-existing poxvirus immunity from recombinant vaccinia immunization. J. Gen. Virol. 2007. 88: 61-70.
Jacquet, A., Vanderschrick, J. F., Vandenbranden, M., Elouahabi, A., Magi, M., Garcia, L. and Ruysschaert, J. M., Vaccination with the recombinant allergen ProDer p 1 complexed with the cationic lipid DiC14-amidine prevents allergic responses to house dust mite. Mol Ther. 2005. 11: 960-968.
Dileo, J., Banerjee, R., Whitmore, M., Nayak, J. V., Fain, L. D. Jr. and Huang, L., Lipid-protamine-DNA-mediated antigen delivery to antigen-presenting cells results in enhanced anti-tumor immune responses. Mol. Ther. 2003. 7: 640-648.
Chen, W., Yan, W. and Huang, L., A simple but effective cancer vaccine consisting of an antigen and a cationic lipid. Cancer Immunol. Immunother. 2008. 57: 517-530.
Vangasseri, D. P., Cui, Z., Chen, W., Hokey, D. A., Falo, L. D. Jr and Huang, L., Immunostimulation of dendritic cells by cationic liposomes. Mol. Membr. Biol. 2006. 23: 385-395.
Yan, W., Chen, W. and Huang, L., Mechanism of adjuvant activity of cationic liposome: Phosphorylation of a MAP kinase, ERK and induction of chemokines. Mol. Immunol. 2007. 44: 3672-3681.
Kawai, T. and Akira, S., TLR signaling. Cell Death Differ. 2006. 13: 816-825.
Gay, N. J. and Gangloff, M., Structure and function of Toll receptors and their ligands. Annu. Rev. Biochem. 2007. 76: 141-165.
Hoebe, K., Du, X., Georgel, P., Janssen, E., Tabeta, K., Kim, S. O., Goode, J. et al., Identification of Lps2 as a key transducer of MyD88-independent TIR signalling. Nature 2003. 424: 743-748.
Kim, H. M., Park, B. S., Kim, J. I., Kim, S. E., Lee, J., Oh, S. C., Enkhbayar, P. et al., Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist Eritoran. Cell 2007. 130: 979-981.
Ohto, U., Fukase, K., Miyake, K. and Satow, Y. Crystal structures of human MD-2 and its complex with antiendotoxic lipid IVa. Science 2007. 316: 1632-1634.
Mata-Haro, V., Cekic, C., Martin, M, Chilton, P. M., Casella, C. R. and Mitchell, T. C., The vaccine adjuvant monophosphoryl lipid A as a TRIF biased agonist of TLR4. Science 2007. 316: 1628-1632.
Schnare, M., Barton, G. M., Holt, A. G., Takeda, K., Akira, S., and Medzhitov, R., Toll-like receptors control activation of adaptive immune responses. Nat. Immunol. 2001. 2: 947-950.
Hoebe, K., Janssen, E. M., Kim, S. O., Alexopoulou, L., Flavell, R. A., Han, J. and Beutler, B., Upregulation of costimulatory molecules induced by lipopolysaccharide and double-stranded RNA occurs by Trif-dependent and Trif-independent pathways. Nat. Immunol. 2003. 4: 1223-1229.
Yamamoto, M., Sato, S., Hemmi, H., Hoshino, K., Kaisho, T., Sanjo, H., Takeuchi, O. et al., Role of adaptor TRIF in the MyD88-independent toll-like receptor signaling pathway. Science 2003. 301: 640-643.
Latz, E., Visintin, A., Lien, E., Fitzgerald, K. A., Monks, B. G., Kurt-Jones, E. A., Golenbock, D. T. et al., Lipopolysaccharide rapidly traffics to and from the Golgi apparatus with the toll-like receptor 4-MD-2-CD14 complex in a process that is distinct from the initiation of signal transduction. J. Biol. Chem. 2002. 277: 47834-47843.
Ruysschaert, J. M., el Ouahabi, A., Willeaume, V., Huez, G., Fuks, R., Vandenbranden, M. and Di Stefano, P., A novel cationic amphiphile for transfection of mammalian cells. Biochem. Biophys. Res. Commun. 1994. 203: 1622-1628.
Lutz, M. B., Kukutsch, N., Ogilvie, A. L., Rossner, S., Koch, F., Romani, N. and Schuler, G., An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow. J. Immunol. Methods 1999. 223: 77-92.
Adam, E., Hansen, K. K., Astudillo Fernandez, O., Coulon, L., Bex, F., Duhant, X., Jaumotte, E. et al., The house dust mite allergen Der p 1, unlike Der p 3, stimulates the expression of interleukin-8 in human airway epithelial cells via a proteinase-activated receptor-2-independent mechanism. J. Biol. Chem. 2006. 281: 6910-6923.