NDS27 combines the effect of curcumin lysinate and hydroxypropyl-β-cyclodextrin to inhibit equine PKCδ and NADPH oxidase involved in the oxidative burst of neutrophils
[en] Polymorphonuclear neutrophils (PMNs) are involved in host defence against infections by the production of reactive oxygen species (ROS), but excessive PMN stimulation is associated with the development of inflammatory diseases. After appropriate stimuli, protein kinase C (PKC) triggers the assembly of NADPH oxidase (Nox2) which produces superoxide anion (O2●-), from which ROS derive. The therapeutic use of polyphenols is proposed to lower ROS production by limiting Nox2 and PKC activities. The purpose of this study was to compare the antioxidant effect of NDS27 and NDS28, two water-soluble forms of curcumin lysinate respectively complexed with hydroxypropyl-β-cyclodextrin (HPβCD) and γ-cyclodextrin (γ-CD), on the activity of Nox2 and PKCδ, involved in the Nox2 activation pathway. Our results, showed that NDS27 is the best inhibitor for Nox2 and PKCδ. This was illustrated by the combined effect of HPβCD and curcumin lysinate: HPβCD, but not γ-CD, improved the release of curcumin lysinate and its exchange against lipid or cholesterol as demonstrated by the lipid coloration with Oil red O, the extraction of radical lipophilic probes recorded by ESR and the HPLC measurements of curcumin. HPβCD not only solubilised and transported curcumin, but also indirectly enhanced its action on both PKC and Nox2 activities. The modulatory effect of NDS27 on the Nox2 activation pathway of neutrophils may open therapeutic perspectives for the control of pathologies with excessive inflammatory reactions.
Research center :
CORD - Centre de l'Oxygène, Recherche et Développement - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Derochette, Sandrine ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Mouithys-Mickalad, Ange ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Franck, Thierry ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Collienne, Simon ; Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale
Ceusters, Justine ; Université de Liège - ULiège > Clinique des grands animaux (chirurgie)
Deby-Dupont, Ginette
Neven, Philippe
Serteyn, Didier ; Université de Liège - ULiège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
NDS27 combines the effect of curcumin lysinate and hydroxypropyl-β-cyclodextrin to inhibit equine PKCδ and NADPH oxidase involved in the oxidative burst of neutrophils
Alternative titles :
[fr] Le NDS27 combine les effets du lysinate de curcumine et de hydroxypropyl-β-cyclodextrine pour inhiber la PKCδ et la NADPH oxydase équine impliquées dans la flambée respiratoire du neutrophile
Publication date :
2014
Journal title :
FEBS Open Bio
eISSN :
2211-5463
Publisher :
Wiley-Blackwell, United States
Volume :
4
Issue :
0
Pages :
1021-1029
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Purification de la NADPH oxydase des neutrophiles équins : mise au point de nouveaux outils pour la quantification et la mesure spécifique de son activité et pour la recherche d'inhibiteurs de l'enzyme
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
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