[en] Natural isolates of Bacillus subtilis are known for their ability to produce a large panel of bioactive compounds. Unfortunately, their recalcitrance to conventional molecular techniques limits their transcript studies. In this work, difficulties to isolate RNA attributed to the cell wall were overcome, finally authorising powerful RT-PCR's.
Disciplines :
Microbiology
Author, co-author :
Guez, Jean-Sebastien
Coutte, Francois
Drucbert, Anne-Sophie
Chihib, Nour-Eddine
Danze, Pierre-Marie
Jacques, Philippe ; Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Language :
English
Title :
Resistance of the cell wall to degradation is a critical parameter for isolation of high quality RNA from natural isolates of Bacillus subtilis.
