hydrogen; biotechnology; Clostridium; dark fermentation; trickle bed; bioreactor
Abstract :
[en] This study investigates the mesophilic biohydrogen production from glucose using a
strictly anaerobic strain, Clostridium butyricum CWBI1009, immobilized in a trickling bed
sequenced batch reactor (TBSBR) packed with a Lantec HD Q-PAC® packing material
(132 ft2/ft3 specific surface). The reactor was operated for 62 days. The main parameters
measured here were hydrogen composition, hydrogen production rate and soluble metabolic
products. pH, temperature, recirculation flow rate and inlet glucose concentration at
10 g/L were the controlled parameters. The maximum specific hydrogen production rate
and the hydrogen yield found from this study were 146 mmol H2/L.d and 1.67 mol H2/mol
glucose. The maximum hydrogen composition was 83%. Following a thermal treatment,
the culture was active without adding fresh inoculum in the subsequent feeding and both
the hydrogen yield and the hydrogen production rate were improved. For all sequences, the
soluble metabolites were dominated by the presence of butyric and acetic acids compared
to other volatile fatty acids. The results from the standard biohydrogen production (BHP)
test which was conducted using samples from TBSBR as inoculum confirmed that the
culture generated more biogas and hydrogen compared to the pure strain of C. butyricum
CWBI1009. The effect of biofilm activity was studied by completely removing (100%) the
mixed liquid and by adding fresh medium with glucose. For three subsequent sequences,
similar results were recorded as in the previous sequences with 40% removal of spent
medium. The TBSBR biofilm density varied from top to bottom in the packing bed and the
highest biofilm density was found at the bottom plates. Moreover, no clogging was evidenced
in this packing material, which is characterized by a relatively high specific surface
area. Following a PCA test, contaminants of the Bacillus genus were isolated and a standard
BHP test was conducted, resulting in no hydrogen production.
Research center :
Centre Wallon de Biologie Industrielle
Disciplines :
Energy Chemical engineering Biochemistry, biophysics & molecular biology Biotechnology
Author, co-author :
Puhulwella, Rathnasiri G.
Beckers, Laurent
Delvigne, Frank ; Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Grigorescu, Alina ; Université de Liège - ULiège > Centre Wallon de biologie industrielle
Thonart, Philippe ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Hiligsmann, Serge ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Language :
English
Title :
Mesophilic biohydrogen production by Clostridium butyricum CWBI1009 in trickling biofilter reactor
Etude de la production d'hydrogène par les bactéries anaérobies chimiotrophes (dark-fermentation)
Funders :
Action de Recherches concertées ARC 07/12 04- ULg- Communauté française FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE] F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] DGTRE - Région wallonne. Direction générale des Technologies, de la Recherche et de l'Énergie [BE]
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