Reference : Matrix ‐ assisted laser desorption/ionization mass spectrometry and Raman spectroscop...
Scientific journals : Short communication
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/171928
Matrix ‐ assisted laser desorption/ionization mass spectrometry and Raman spectroscopy: An interesting complementary approach for lipid detection in biological tissues
English
Jadoul, Laure* mailto [Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.) >]
Malherbe, Cédric* mailto [Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique >]
calligaris, David []
Longuespée, Rémi mailto [Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.) >]
Gilbert, Bernard mailto [Université de Liège - ULiège > Département de chimie (sciences) > Département de chimie (sciences) >]
Eppe, Gauthier mailto [Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique >]
De Pauw, Edwin mailto [Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.) >]
* These authors have contributed equally to this work.
2014
European Journal of Lipid Science and Technology
Wiley-VCH Verlag
Yes (verified by ORBi)
International
1438-7697
1438-9312
Weinheim
Germany
[en] Glycerophospholipids ; MALDI MSI ; Raman spectroscopy
[en] Recently, matrix‐assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) has emerged as a powerful technique to study the distribution of lipids. However, quantification still remains a challenge because the MALDI signal is strongly affected by ion suppression effects. On the contrary, Raman spectroscopy is recognized as a non‐destructive analysis method and spectral images can also be acquired. The combination of these two techniques was applied for lipids detection in tissue sections. In MALDI, two lipids families (glycerophosphocholine, PC; gycerophosphoethanolamine, PE), three MALDI matrices (1,5‐diaminonapthalene, 1,5‐DAN; 2,5‐dihydroxybenzoic acid, 2,5‐DHB; a‐4‐hydroxicinammic acid, CHCA), and various mixtures of lipids were investigated. The nature of the lipid, as well as the nature of the matrix and the composition of the sample influences the signal of a given lipid. In Raman, despite a strong overlap with the spectrum of the native tissue, an intensity profile constructed along the diameter of the section clearly shows that the signature of one given lipid (a glycerophosphocholine) can be detected on a doped biological sample.
Mass Spectrometry Laboratory, Department of Chemistry, University of Liège, Liège, Belgium ; Inorganic Analytical Chemistry Laboratory, Department of Chemistry, University of Liège, Liège, Belgium
http://hdl.handle.net/2268/171928
10.1002/ejlt.201300198

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