[en] Decalactones are interesting flavouring compounds that can be produced from ricinoleic acid. In this study, the production of lactones in biofilms using Yarrowia lipolytica is investigated. The hydrophobia of cells increased for increased aeration rates resulting in higher adhesion when the reactor wall was hydrophobic (plastic). To increase adhesion, sheets of methyl-polymethacrylate (PMMA) were added in the reactor and the production of lactones increased with the surface of plastic added, reaching 850 mg/L of 3-hydroxy- -decalactone for 60 cm2. In an Airlift bioreactor made of PMMA, biofilms were present at the top of the reactor for increased aeration. In the meantime, a metabolic shift occurred resulting in high amounts of 3-hydroxy- -decalactone. At 0.493 vvm and 61 h of culture, the dissolved oxygen ratio was of 28.6% and cells grew to only 1.29 × 106 cells/mL in the liquid medium but 3-hydroxy -decalactone
accumulated to 1.7 g/L instead of less than 0.3 mg/L for lower aeration. Adhering cells had a particular elongated shape intermediate between the yeast and the pseudofilamentous forms. It is concluded that adhering Y. lipolytica cells are in a specific physiological state changing their structure but also their metabolic properties and these properties make them good candidate for simple immobilisation process.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Escamilla Garcia, E.; AgroSup Dijon & University of Burgundy, 1, Esplanade Erasme, 21000 Dijon, France > Laboratory of Microbial and Food Process (PAM), Joint Laboratory
O’Riordan, S.; AgroSup Dijon & University of Burgundy, 1, Esplanade Erasme, 21000 Dijon, France > Laboratory of Microbial and Food Process (PAM), Joint Laboratory,
Gomes, N.; Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal > IBB-Institute for Biotechnology and Bioengineering
Aguedo, Mario ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Belo, I.; Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal > IBB-Institute for Biotechnology and Bioengineering
Teixeira, J.; Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal > IBB-Institute for Biotechnology and Bioengineering
Belin, J.-M.; AgroSup Dijon & University of Burgundy, 1, Esplanade Erasme, 21000 Dijon, France > Laboratory of Microbial and Food Process (PAM), Joint Laboratory
Waché, Y.; AgroSup Dijon & University of Burgundy, 1, Esplanade Erasme, 21000 Dijon, France > Laboratory of Microbial and Food Process (PAM), Joint Laboratory
Language :
English
Title :
An air-lift biofilm reactor for the production of g-decalactones by Yarrowia lipolytica
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