Fragmentation and isomerization due to field heating in traveling wave ion mobility

[en] During their travel inside a traveling wave ion mobility cell (TW IMS), ions are susceptible to heating because of the presence of high intensity electric fields. Here, we report effective temperatures T eff,vib obtained at the injection and inside the mobility cell of a SYNAPT G2 HDMS spectrometer for different probe ions: benzylpyridinium ions and leucine enkephalin. Using standard parameter sets, we obtained a temperature of ~800 K at injection and 728 ± 2 K into the IMS cell for p-methoxybenzylpyridinium. We found that T eff,vib inside the cell was dependent on the separation parameters and on the nature of the analyte. While the mean energy of the Boltzmann distributions increases with ion size, the corresponding temperature decreases because of increasing numbers of vibrational normal modes. We also investigated conformational rearrangements of 7+ ions of cytochrome c and reveal isomerization of the most compact structure, therefore highlighting the effects of weak heating on the gas-phase structure of biologically relevant ions.

Research Center/Unit :

Giga-Systems Biology and Chemical Biology - ULiège

Disciplines :

Chemistry

Author, co-author :

Morsa, Denis ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)

Gabelica, Valérie ; Inserm & Univ. Bordeaux, F-33600, Pessac, France > U869 - ARNA Laboratory,

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)

Language :

English

Title :

Fragmentation and isomerization due to field heating in traveling wave ion mobility

Publication date :

2014

Journal title :

Journal of the American Society for Mass Spectrometry

ISSN :

1044-0305

eISSN :

1879-1123

Publisher :

Elsevier Science, New York, United States - New York

Volume :

Issue :

Pages :

1384-1393

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://link.springer.com/article/10.1007%2Fs13361-014-0909-9

Name of the research project :

Denis Morsa Thesis

Funders :

FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture
F.R.S.-FNRS - Fonds de la Recherche Scientifique

Available on ORBi :

since 22 August 2014

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