[en] Molecular biology techniques such as PCR constitute powerful tools for the determination of the taxonomic origin of bones. DNA degradation and contamination by exogenous DNA, however, jeopardise bone identification. Despite the vast array of techniques used to decontaminate bone fragments, the isolation and determination of bone DNA content are still problematic. Within the framework of the eradication of transmissible spongiform encephalopathies (including BSE, commonly known as "mad cow disease"), a fluorescence in situ hybridization (FISH) protocol was developed. Results from the described study showed that this method can be applied directly to bones without a demineralisation step and that it allows the identification of bovine and ruminant bones even after severe processing. The results also showed that the method is independent of exogenous contamination and that it is therefore entirely appropriate for this application.
Research center :
Centre wallon de Recherches agronomiques
Disciplines :
Food science Veterinary medicine & animal health Animal production & animal husbandry Agriculture & agronomy
Author, co-author :
Lecrenier, Marie-Caroline ✱; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Epidémiologie et analyse des risques appl. aux sc. vétér.
Ledoux, Quentin ✱; Centre wallon de Recherches agronomiques > Département Valorisation des productions > Unité Qualité des produits
Berben, Gilbert; Centre wallon de Recherches agronomiques > Département Valorisation des productions > Unité Authentification et traçabilité
Fumière, Olivier; Centre wallon de Recherches agronomiques > Département Valorisation des productions > Unité Authentification et traçabilité
Saegerman, Claude ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Epidémiologie et analyse des risques appl. aux sc. vétér.
Baeten, Vincent; Centre wallon de Recherches agronomiques > Département Valorisation des productions > Unité Qualité des produits
Veys, Pascal; Centre wallon de Recherches agronomiques > Département Valorisation des productions > Unité Qualité des produits
✱ These authors have contributed equally to this work.
Language :
English
Title :
Determination of the ruminant origin of bone particles using fluorescence in situ hybridization (FISH).
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