Date; Btana; Traditional Food; Osmophilic flora; Leuconostoc; Antibacterial activity
Abstract :
[en] The current study aimed to isolate and identify dominant osmophilic bacteria associated with a traditional date product named “Btana”, produced in south region of Maghreb countries. Samples were randomly collected after two month of storage from tow villages (Mtarfat and Abani) in the Algerian southern department “Adrar”. A high osmotic pressure medium (MSE) was used for isolation of osmophilic bacteria, which were purified and examined for macroscopic and microscopic shape, Gram stain, catatalse, oxydase, acetoine and ADH production, reduction of nitrate, and motility. Isolates were then subculture on MRS medium for production of dextran, gas from glucose, growth in the presence of NaCl (3, 6.5 %) and sucrose (10, 20, 30, 40, and 50 %), pH tolerance (4.8, 6.5), growth temperature (10, 37, and 45°C) and thermo resistance (55°C for 15 min), enzymatic activity (proteolytic, lipolytic, hemolysis). Isolates were identified to specie’s level by sugar fermentation. Their growth and acidification kinetic were also studied. Results identified two species of Leuconostoc; Leuconostoc mesenteroides subsp. mesenteroides and Leuconostoc mesenteroides subsp. dextranicum. They show a high antibacterial activity against four indicator bacteria; Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923.
Disciplines :
Microbiology
Author, co-author :
Abekhti, Abdelkader; Univesity of Biskra > Department of Nature and life science
Daube, Georges ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Kihal, M.; Oran University > Faculty of Science > Laboratory of Applied Microbiology, Department of Biology
Language :
English
Title :
Isolation and identification of dominant osmophilic Leuconostoc strains from traditional date product “Btana”
Publication date :
2014
Journal title :
International Food Research Journal
ISSN :
1985-4668
eISSN :
2231-7546
Publisher :
Universiti Putra Malaysia. Faculty of Food Science and Technology, Selangor, Malaysia
Abekhti, A., Zarour, K., Boulal, A., Benmechernene, Z. and Kihal, M. 2013. Evaluation of Microbiological Quality of the Date Fruit Product "Btana" Produced in Adrar South Algeria. Journal of Microbiology Research 3(5): 163-170.
Allane, T. and Benamara, S. 2010. Activite´s antioxydantes de quelques fruits communs et sauvages d'Alge´rie. Phytothe´rapie 3(8): 171-175.
Antunes, A., Rainey, F. A., Nobre, M. F., Schumann, P., Ferreira. A. M., Ramos. A., Santos, H. and Costa, M. 2002. Leuconostoc ficulneum sp. nov., a novel lactic acid bacterium isolated from a ripe fig, and reclassification of Lactobacillus fructosus as Leuconostoc fructosum comb. nov. International Journal of Systematic and Evolutionary Microbiology 52 (2): 647-655.
Barrangou, R., Yoon, S. S., Breidt, F., Fleming, H. P. and Klaenhammer, T. R. 2002. Characterization of six Leuconostoc fallax bacteriophages isolated from an industrial sauerkraut fermentation. Applied and Environmental Microbiology 68 (11): 5452-5458.
Bellengier, P., Richard, J. and Foucaud, C. 1997. Nutritional requirements of Leuconostoc mesenteroides subsp. mesenteroides and subsp. dextranicum for growth in milk. Journal of Dairy Research 64: 95-103.
Bjo¨rkroth, J. and Holzapfel, W. 2006. Genera Leuconostoc, Oenococcus and Weissella. In M. Dworkin, S., Falkow, E., Rosenberg, K., Schleifer, H. and Stackebrandt E. (Eds). The Prokaryotes (4), p. 267-319. New York: Springer.
Bonomo, M. G., Salzano, G. and Ricciardi, A. 2010. Influence of autochthonous starter cultures on microbial dynamics and chemical-physical features of traditional fermented sausages of Basilicata region. World Journal of Microbiology and Biotechnology 27 (1): 137-146.
Buffa, M., Morais, J., Jime´nez-Belenguer, A., Herna´ndez-Gimenez, E. and Guamis, B. 2005. Technological characterization of lactic acid bacteria isolated from raw ewes' milk for cheese making. Milchwissenschaft 61 (4): 404-407.
Devoyod, J. J. and Poullain, F. 1988. The Leuconostocs, Characteristics, Their role in dairy technology. Lait 68 (3): 249-280.
Dols, M., Chrabi, W., Remaud-Simeon, M., Lindley, N. D. and Monsan, P. 1997a. Growth and energetics of Leuconostoc mesenteroides NRRL B-1299 during metabolism of various sugars and their consequences for dextransucrase production. Applied and Environmental Microbiology 63 (61): 2159-2165.
Ehrmann, M. A., Freiding., S. and Vogel, R. F. 2009. Leuconostoc palmae sp. nov., a novel lactic acid bacterium isolated from palm wine. International Journal of Systematic and Evolutionary Microbiology 59 (5): 943-947.
Elleuch, M., Besbes, S., Roiseux, O., Blecker, C., Deroanne, C., Drira, N. E. and Attia, H. 2008. Date flesh: Chemical composition and characteristics of the dietary fiber. Food Chemistry 11 (3): 676-682.
Farrow, J. A. E., Facklam, R. F. and Collins, M. D. 1989. Nucleic acid homologies of some vancomycin-resistant Leuconostocs and description of Leuconostoc citreum sp. nov. and Leuconostoc pseudomesenteroides sp. nov. International Journal of Systematic and Bacteriology 39:279-283.
Galvez, A. A., Dubois-Dauphin, R., Ghalfi, H., Campos, D. and Thonart, P. 2009. Description of two Enterococcus strains isolated from traditional Peruvian artisanal-produced cheeses with a bacteriocin-like inhibitory activity. Biotechnology Agronomy Society and Environment 13 (3): 349-356.
Garvie, E. I. 1986. Genus Leuconostoc van Tieghem 1878. In Sneath, P. H. A., Mair, N. S., Sharpe, M. E. and Holt J. G. (Eds). Bergey's Manual of Systematic Bacteriology 2, p. 1071-1075. Baltimore: Williams and Wilkins.
Ghazi, F., Henni, D. E., Benmchernene, Z. and Kihal, M. 2009. Phenotypic and whole cell protein analysis by SDS-PAGE for identification of dominants lactic acid bacteria isolated from Algerian raw milk. World Journal of Dairy Food Science 4 (1): 78-87.
Hadadji, M., Benama, R., Saidi, N., Henni, D. E. and Kihal, M. 2005. Identification of cultivable Bifidobacterium species isolated from breast-fed infants feces in West-Algeria. African Journal of Biotechnology 4 (5): 422-430.
Harun-ur-Rashid, M., Togo, K., Ueda, M. and Miyamoto, T. 2007. Identification and characterization of dominant lactic acid bacteria isolated from traditional fermented milk Dahi in Bangladesh. World Journal of Microbiology and Biotechnology 23 (1): 125-133.
Hasnaoui, A., Elhoumaizi, M. A., Asehraou, A., Sindic, D. M. and Hakkou, A. 2010. Chemical composition and microbial quality of the main varieties of dates grown in Figuig oasis of Morocco. International journal of agriculture and biology 12 (2): 311-314.
Jamuna, M., Babusha, S. T. and Jeevaratnam, K. 2005. Inhibitory efficacy of nisin and bacteriocins from Lactobacillus isolates against food spoilage and pathogenic organisms in model and food systems. Food Microbiology 22 (5): 449-454.
Jespersen, L. 2003. Occurrence and taxonomic characteristics of strains of Saccharomyces cerevisiae predominant in African indigenous fermented foods and beverages. Yeast Research 3 (2): 191-200.
Juste´, A., Lievens, B., Frans, I., Klingeberg, M., Michiels, C. W. and Willems, K. A. 2008d. Present knowledge of the bacterial micro flora in the extreme environment of sugar thick juice. Food Microbiology 25 (6): 831-836.
Kaletunc, G., Lee, J., Alpas, H. and Bozoglu, F. 2004. Evaluation of structural changes induced by high hydrostatic pressure in Leuconostoc mesenteroides. Applied and Environmental Microbiology 70 (2): 1116-1122.
Levata-Jovanovic, M. and Sandine, W. E. 1996. Citrate Utilization and Diacetyl Production by Various Strains of Leuconostoc mesenteroides ssp. cremoris. Journal of Dairy Science 79 (11):1928-1935.
Mami, A., Henni J. E. and Kihal, M. 2008. Antimicrobial Activity of Lactobacillus species isolated from Algerian raw goat's milk against Staphylococcus aureus. World Journal of Dairy and Food Sciences 3 (2): 39-49.
Mayeux, J. V., Sandine, W. E. and Elliker, P. R. 1962. A selective medium for detecting Leuconostoc organisms in mixed-strain starter cultures. Journal of Dairy Science 45: 655-656.
Nieto-Arribas, P., Sesen, S., Palop, L. and Cabezas, L. 2010. Genotypic and technological characterization of Leuconostoc isolates to be used as adjunct starters in Manchego cheese manufacture. Food Microbiology 27 (1): 85-93.
Nuñez, M. and M. Medina. 1979. La flore lactique du fromage bleu de Cabrales. Lait 59: 497-513.
Ouadghiri, M., Amar, M., Vancanneyt, M. and Swings, J. 2005. Biodiversity of lactic acid bacteria in Moroccan soft white cheese (Jben). Microbiology Letters 251 (2): 267-271.
Sawale, S.D. and Lele, S. S. 2010. Statistical Optimization of Media for Dextran Production by Leuconostoc sp., Isolated from Fermented Idli Batter. Food Science and Biotechnology 19(2): 471-478.
Server-Busson, C., Danzart, M., Juillard, V. and Leveau, J. Y. 1998. Characterization and selection of dairy Lactococci based on statistical analyses of biochemical and technological properties. Lait 78: 543-556
Stiles, M. E. 1994. Bacteriocins produced by Leuconostoc species. Journal of Dairy Science 77 (9): 2718-2724.
Vayalil, P. K. 2012. Date fruits (Phoenix dactylifera Linn): an emerging medicinal food. Critical Reviews in Food Science and Nutrition 52 (3):249-71.
Vihavainen, E. J., Murros, A. E. and Bjo¨rkroth, K. J. 2008. Leuconostoc spoilage of vacuum-packaged vegetable sausages. Journal of Food Protection 71 (11): 2312-2315.
Xiraphi, N., Georgalaki, M., Rantsiou, K., Cocolin, L., Tsakalidou, E. and Drosinos, E. H. 2008. Purification and characterization of a bacteriocin produced by Leuconostoc mesenteroides E131. Meat Science 80 (2): 194-203.
