level set method; curvature based growth; tissue engineering; scaffold design
Abstract :
[en] Three dimensional (3D) open porous scaffolds are commonly used in tissue engineering (TE) applications to provide an initial template for cell attachment and subsequent cell growth and construct development. The macroscopic geometry of the scaffold is key in determining the kinetics of cell growth and thus in vitro ‘tissue’ formation. In this study we developed a computational framework based on the level set methodology to predict curvature-dependent growth of the cell/extracellular matrix domain within TE constructs. Scaffolds with various geometries (hexagonal, square, triangular) and pore sizes (500 and 1000 µm) were produced in house by additive manufacturing, seeded with human periosteum-derived cells and cultured under static conditions for 14 days. Using the projected tissue area as an output measure, the comparison between the experimental and the numerical results demonstrated a good qualitative and quantitative behavior of the framework. The model in its current form is able to provide important spatio-temporal information on final shape and speed of pore-filling of tissue engineered constructs by cells and extracellular matrix during static culture.
Disciplines :
Engineering, computing & technology: Multidisciplinary, general & others
Author, co-author :
Guyot, Yann ; Université de Liège - ULiège > Département d'aérospatiale et mécanique > Génie biomécanique
papantoniou, Ioannis; KULeuven > Skeletal Biology and Engineering Research Center
Chai, Yoke Chin; KULeuven > Skeletal Biology and Engineering Research Center
Van Bael, Simon; Katholieke Universiteit Leuven - KUL > Division of Production engineering
Schrooten, Jan; Katholieke Universiteit Leuven - KUL > Departement of Mechanical engineering
Geris, Liesbet ; Université de Liège - ULiège > Département d'aérospatiale et mécanique > Génie biomécanique
Language :
English
Title :
A computational model for cell/ECM growth on 3D surfaces using the level set method: a bone tissue engineering case study
Publication date :
April 2014
Journal title :
Biomechanics and Modeling in Mechanobiology
ISSN :
1617-7959
eISSN :
1617-7940
Publisher :
Springer, Germany
Peer reviewed :
Peer Reviewed verified by ORBi
European Projects :
FP7 - 279100 - BRIDGE - Biomimetic process design for tissue regeneration: from bench to bedside via in silico modelling
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] CER - Conseil Européen de la Recherche [BE] FWO - Fonds Wetenschappelijk Onderzoek Vlaanderen [BE] CE - Commission Européenne [BE]
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