Poster (Scientific congresses and symposiums)
Studies of the Domains II and III of Bacillus subtilis PBP4a in relation with the protein localization
Vanden Broeck, Arnaud; Van Der Heiden, Edwige; Sauvage, Eric et al.
2014IAP Day - iPROS
 

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Keywords :
Bacillus; PBP; Cellular localization
Abstract :
[en] Bacillus subtilis PBP4a belongs to the class-C1 PBPs characterized by two internal additional domains of unknown function. Seven lysine residues (K) are protruding from domain II. Four of them have been mutated in glutamine residues (Q). Both proteins (WT and Mut4KQ PBP4a) have been produced without signal peptide in E. coli and their sub-cellular localizations determined by measuring the DD-carboxypeptidase activities in the different compartments (cytoplasmic vs membrane attached proteins). In order to detect a possible influence of the PBP4a domain III in the localization of the protein, its encoding sequence has been cloned into pET-28b-BlaP, a vector allowing the production of WT BlaP β-lactamase or BlaP/DIII chimeric protein (with domain III inserted in a permissive loop of BlaP). The nitrocefin hydrolysis activities of BlaP or BlaP/DIII have been measured in the different cellular compartments.
Research center :
Centre for Protein Engineering
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Vanden Broeck, Arnaud ;  Université de Liège - ULiège > 2e an. master bioch. & biol. moléc. & cell., fin. appr.
Van Der Heiden, Edwige ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Sauvage, Eric ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Rhazi, Noureddine
Huynen, Céline ;  Université de Liège - ULiège > Département des sciences de la vie > Enzymologie et repliement des protéines
Verlaine, Olivier ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Joris, Bernard ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
Duez, Colette ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Language :
English
Title :
Studies of the Domains II and III of Bacillus subtilis PBP4a in relation with the protein localization
Publication date :
23 April 2014
Number of pages :
A0
Event name :
IAP Day - iPROS
Event organizer :
uGhent
Event place :
Ghent, Belgium
Event date :
23rd of April 2014
Funders :
Belgian Science Policy : IAP n° P6/19 and P7/44
Available on ORBi :
since 29 April 2014

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