Article (Scientific journals)
Comparison Of Yarrowia Lipolytica Lipase Immobilization Yield Of Entrapment, Adsorption, And Covalent Bond Techniques
Alloue, Wam.; Destain, Jacqueline; El Mejdoub, Thami et al.
2008In Applied Biochemistry and Biotechnology, 150 (1), p. 51-63
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Keywords :
Yarrowia lipolytica lipase; Enzymatic activity; Immobilization; Reusability
Abstract :
[en] The purpose of this study was to immobilize lipase from Yarrowia lipolytica using three methods including inclusion, adsorption, and covalent bond to study enzyme leaching, storage, and catalytic properties. Sodium alginate and chitosan were the polymers selected to immobilize lipase by inclusion. The beads of each polymer were dried by freeze drying and fluidization. The results show that chitosan was more adapted to the inclusion of lipase. Even though freeze dried, bead activity was low compared to that of fluidized beads. The freeze-drying process seems to produce suitable beads for storage at 4 and 20 °C. The immobilization by adsorption was carried out on both celite and silica gel. Maximum immobilization yield of 76% was obtained with celite followed by 43% in silica gel. The enzyme adsorbed on the two supports exhibited greater stability at a certain temperature (50 °C) and in no polar solvents (Isooctane, n-heptane, and n-hexane). In addition, the lipase immobilized by covalent bond retained residual activity equitable to 70%. It was demonstrated that the enzyme immobilized by covalent bond showed greater activity (80%) after 5 months of storage.
Disciplines :
Microbiology
Author, co-author :
Alloue, Wam.
Destain, Jacqueline ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
El Mejdoub, Thami ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Ghalfi, H.
Kabran, P.
Thonart, Philippe ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles
Language :
English
Title :
Comparison Of Yarrowia Lipolytica Lipase Immobilization Yield Of Entrapment, Adsorption, And Covalent Bond Techniques
Publication date :
2008
Journal title :
Applied Biochemistry and Biotechnology
ISSN :
0273-2289
eISSN :
1559-0291
Publisher :
Humana Press, United States - New Jersey
Volume :
150
Issue :
1
Pages :
51-63
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 20 March 2014

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