Article (Scientific journals)
An enhanced process for the production of a highly purified extracellular lipase in the non-conventional yeast Yarrowia lipolytica
Turki, S.; Ayed, A.; Chalghoumi, N. et al.
2010In Applied Biochemistry and Biotechnology, 160 (5), p. 1371-1385
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Keywords :
Bioreactor; Fed-batch culture; Lipase; Purification; Yarrowia lipolytica; Anion exchange chromatography; Batch culture; Batch process; Economic production
Abstract :
[en] Yarrowia lipolytica LgX64.81 is a non-genetically modified mutant that was previously identified as a promising microorganism for extracellular lipase production. In this work, the development of a fed-batch process for the production of this enzyme in this strain was described. A lipolytic activity of 2,145 U/mL was obtained after 32 h of batch culture in a defined medium supplemented with 10 g/L of tryptone, an enhancer of lipase expression. To maximize the volumetric productivity, two different fed-batch strategies had been investigated. In comparison to batch process, the intermittent fed-batch strategy had not improved the volumetric lipase productivity. In contrast, the stepwise feeding strategy combined with uncoupled cell growth and lipase production phases resulted in a 2-fold increase in the volumetric lipase productivity, namely, the lipase activity reached 10,000 U/mL after 80 h of culture. Furthermore, this lipase was purified to homogeneity by anion exchange chromatography on MonoQ resin followed by gel filtration on Sephacryl S-100. This process resulted in an overall yield of 72% and a 3.5-fold increase of the specific lipase activity. The developed process offers a great potential for an economic production of Lip2 at large scale in Y. lipolytica LgX64.81. © 2009 Humana Press.
Disciplines :
Microbiology
Author, co-author :
Turki, S.;  Unité de Fermentation, Institut Pasteur de Tunis, 13, place Pasteur BP 74, Tunis Belvédère 1002, Tunisia
Ayed, A.;  Unité de Fermentation, Institut Pasteur de Tunis, 13, place Pasteur BP 74, Tunis Belvédère 1002, Tunisia
Chalghoumi, N.;  Unité de Fermentation, Institut Pasteur de Tunis, 13, place Pasteur BP 74, Tunis Belvédère 1002, Tunisia
Weekers, F.;  Artechno S.A., Rue François Bovesse, Gembloux 1. 5030, Belgium
Thonart, Philippe ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Kallel, H.;  Unité de Fermentation, Institut Pasteur de Tunis, 13, place Pasteur BP 74, Tunis Belvédère 1002, Tunisia
Language :
English
Title :
An enhanced process for the production of a highly purified extracellular lipase in the non-conventional yeast Yarrowia lipolytica
Publication date :
2010
Journal title :
Applied Biochemistry and Biotechnology
ISSN :
0273-2289
eISSN :
1559-0291
Publisher :
Humana Press, United States - New Jersey
Volume :
160
Issue :
5
Pages :
1371-1385
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 20 March 2014

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