Reference : Lack of isocitrate lyase in Chlamydomonas leads to changes in carbon metabolism and i...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/163291
Lack of isocitrate lyase in Chlamydomonas leads to changes in carbon metabolism and in the response to oxidative stress under mixotrophic growth.
English
Plancke, Charlotte []
Vigeolas, Hélène [Université de Liège - ULiège > Département des sciences de la vie > Génétique >]
Hohner, Ricarda [> >]
Roberty, Stéphane mailto [Université de Liège - ULiège > Département des sciences de la vie > Génétique >]
Emonds-Alt, Barbara mailto [Université de Liège - ULiège > Département des sciences de la vie > Génétique >]
Larosa, Véronique mailto [Université de Liège - ULiège > Département des sciences de la vie > Génétique >]
Willamme, Rémi mailto [Université de Liège - ULiège > Département des sciences de la vie > Génétique et Physiologie des Microalgues > >]
Duby, Franceline mailto [Université de Liège - ULiège > Département des sciences de la vie > Génétique >]
Onga Dhali, David [> >]
Thonart, Philippe mailto [Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Hiligsmann, Serge mailto [Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Franck, Fabrice mailto [Université de Liège - ULiège > > Labo de Bioénergétique >]
Eppe, Gauthier mailto [Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique >]
Cardol, Pierre mailto [Université de Liège - ULiège > Département des sciences de la vie > Génétique et Physiologie des Microalgues > >]
Hippler, Michael [> >]
Remacle, Claire mailto [Université de Liège - ULiège > Département des sciences de la vie > Génétique et Physiologie des Microalgues > >]
2014
The Plant journal : for cell and molecular biology
77
3
404-417
Yes (verified by ORBi)
International
0960-7412
1365-313X
[en] Chlamydomonas ; carbon metabolism ; glyoxylate cycle ; isocitrate lyase ; mixotrophic growth ; mutant ; oxidative stress
[en] Isocitrate lyase is a key enzyme of the glyoxylate cycle. This cycle plays an essential role in cell growth on acetate, and is important for gluconeogenesis as it bypasses the two oxidative steps of the tricarboxylic acid (TCA) cycle in which CO2 is evolved. In this paper, a null icl mutant of the green microalga Chlamydomonas reinhardtii is described. Our data show that isocitrate lyase is required for growth in darkness on acetate (heterotrophic conditions), as well as for efficient growth in the light when acetate is supplied (mixotrophic conditions). Under these latter conditions, reduced acetate assimilation and concomitant reduced respiration occur, and biomass composition analysis reveals an increase in total fatty acid content, including neutral lipids and free fatty acids. Quantitative proteomic analysis by 14 N/15 N labelling was performed, and more than 1600 proteins were identified. These analyses reveal a strong decrease in the amounts of enzymes of the glyoxylate cycle and gluconeogenesis in parallel with a shift of the TCA cycle towards amino acid synthesis, accompanied by an increase in free amino acids. The decrease of the glyoxylate cycle and gluconeogenesis, as well as the decrease in enzymes involved in beta-oxidation of fatty acids in the icl mutant are probably major factors that contribute to remodelling of lipids in the icl mutant. These modifications are probably responsible for the elevation of the response to oxidative stress, with significantly augmented levels and activities of superoxide dismutase and ascorbate peroxidase, and increased resistance to paraquat.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/163291
10.1111/tpj.12392
(c) 2013 The Authors The Plant Journal (c) 2013 John Wiley & Sons Ltd.

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Plancke et al.pdfPublisher postprint664.48 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.