[en] We reported previously on the function of acyl coenzyme A (acyl-CoA) oxidase isozymes in the yeast Yarrowia lipolytica by investigating strains disrupted in one or several acyl-CoA oxidase-encoding genes (POX1 through POX5) (H. Wang et al., J. Bacteriol. 181:5140-5148, 1999). Here, these mutants were studied for lactone production. Monodisrupted strains produced similar levels of lactone as the wild-type strain (50 mg/liter) except for Δpox3, which produced 220 mg of γ-decalactone per liter after 24 h. The Δpox2 Δpox3 double-disrupted strain, although slightly affected in growth, produced about 150 mg of lactone per liter, indicating that Aox2p was not essential for the biotransformation. The Δpox2 Δpox3 Δpox5 triple- disrupted strain produced and consumed lactone very slowly. On the contrary, the Δpox2 Δpox3 Δpox4 Δpox5 multidisrupted strain did not grow or biotransform methyl ricinoleate into γ-decalactone, demonstrating that Aox4p is essential for the biotransformation.
Disciplines :
Biotechnology
Author, co-author :
Waché, Y.; Laboratoire de Biotechnologie, Ecl. Natl. Sup. Biol. Appl. Nutr. A., Université de Bourgogne, 21000 Dijon, France, Laboratoire de Biotechnologie, Ecl. Natl. Sup. Biol. Appl. Nutr. A., Université de Bourgogne, 1, esplanade Erasme, 21000 Dijon, France
Laroche, C.; Laboratoire de Biotechnologie, Ecl. Natl. Sup. Biol. Appl. Nutr. A., Université de Bourgogne, 21000 Dijon, France
Bergmark, K.; Laboratoire de Biotechnologie, Ecl. Natl. Sup. Biol. Appl. Nutr. A., Université de Bourgogne, 21000 Dijon, France
Møller-Andersen, C.; Laboratoire de Biotechnologie, Ecl. Natl. Sup. Biol. Appl. Nutr. A., Université de Bourgogne, 21000 Dijon, France
Aguedo, Mario ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Le Dall, M.-T.; Lab. de Genet. des Micro-organismes, INRA-CNRS, URA1925, 78850 Thiverval-Grignon, France
Wang, H.; Lab. de Genet. des Micro-organismes, INRA-CNRS, URA1925, 78850 Thiverval-Grignon, France
Nicaud, J.-M.; Lab. de Genet. des Micro-organismes, INRA-CNRS, URA1925, 78850 Thiverval-Grignon, France
Belin, J.-M.; Laboratoire de Biotechnologie, Ecl. Natl. Sup. Biol. Appl. Nutr. A., Université de Bourgogne, 21000 Dijon, France
Language :
English
Title :
Involvement of acyl coenzyme A oxidase isozymes in biotransformation of methyl ricinoleate into γ-decalactone by Yarrowia lipolytica
Publication date :
2000
Journal title :
Applied and Environmental Microbiology
ISSN :
0099-2240
eISSN :
1098-5336
Publisher :
American Society for Microbiology, Washington, United States - District of Columbia
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