Increased electron donor and electron acceptor characters enhance the adhesion between oil droplets and cells of Yarrowia lipolytica as evaluated by a new cytometric assay
Aguedo, Mario; Waché, Y.; Mazoyer, V.et al.
2003 • In Journal of Agricultural and Food Chemistry, 51 (10), p. 3007-3011
[en] The adhesion of methyl ricinoleate droplets to cells of the yeast Yarrowia lipolytica was investigated. A new cytometric method, relying on the double staining of fatty globules with Nile Red and of cells with Calcofluor, enabled us to quantify methyl ricinoleate droplet adhesion to cells precultured on a hydrophilic or on a hydrophobic carbon source. In this last case, droplet adsorption was enhanced and a MATS (microbial adhesion to solvents) test revealed that this increase was due to Lewis acid-base interactions and not to an increase in the hydrophobic properties of the cell surface. These preliminary results demonstrate that the developed cytometric method is promising for various applications concerning the study of interactions between microorganisms and an emulsified hydrophobic substrates.
Disciplines :
Biotechnology
Author, co-author :
Aguedo, Mario ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Waché, Y.; Laboratoire de Microbiologie, UMR UB/INRA 1082, ENSBANA, 21 000 Dijon, France
Mazoyer, V.; Laboratoire de Microbiologie, UMR UB/INRA 1082, ENSBANA, 21 000 Dijon, France
Sequeira-Le Grand, A.; Centre de Cytometrie en Flux, Université de Bourgogne, 21 000 Dijon, France
Belin, J.-M.; Laboratoire de Microbiologie, UMR UB/INRA 1082, ENSBANA, 21 000 Dijon, France
Language :
English
Title :
Increased electron donor and electron acceptor characters enhance the adhesion between oil droplets and cells of Yarrowia lipolytica as evaluated by a new cytometric assay
Publication date :
2003
Journal title :
Journal of Agricultural and Food Chemistry
ISSN :
0021-8561
eISSN :
1520-5118
Publisher :
American Chemical Society, Washington, United States - District of Columbia
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