Reference : Partial characterization of testosterone-metabolizing enzymes in the quail brain.
Scientific journals : Article
Life sciences : Zoology
Social & behavioral sciences, psychology : Neurosciences & behavior
Partial characterization of testosterone-metabolizing enzymes in the quail brain.
Schumacher, M. [> > > >]
Contenti, E. [> > > >]
Balthazart, Jacques mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau >]
Brain Research
Yes (verified by ORBi)
[en] 3-Oxo-5-alpha-Steroid 4-Dehydrogenase/metabolism ; Animals ; Aromatase/metabolism ; Brain/enzymology ; Coturnix/metabolism ; Dihydrotestosterone/biosynthesis ; Estradiol/biosynthesis ; Kinetics ; Oxidoreductases/metabolism ; Quail/metabolism ; Temperature ; Testosterone/metabolism
[en] The properties of 5 beta-reductase, 5 alpha-reductase and aromatase, 3 testosterone metabolizing enzymes, were studied in the quail brain by an in vitro incubation technique. The results describe the changes in time of metabolite production and the effects of temperature, enzyme and cofactor concentrations. The apparent Km and Vmax were evaluated for the 3 enzymes. Aromatase and 5 alpha-reductase have a higher affinity but a lower capacity than 5 beta-reductase. The kinetics of the latter enzyme are complex and suggest the presence of two types of enzymes. These characteristics fit in well with the role probably played by the enzymes in vivo.

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