Enzymes from Cold-Adapted Microorganisms. The Class C Beta-Lactamase from the Antarctic Psychrophile Psychrobacter Immobilis A5

Feller, Georges; Zekhnini, Z.; Lamotte-Brasseur, Josette; Gerday, Charles

doi:10.1111/j.1432-1033.1997.00186.x

Article (Scientific journals)

Enzymes from Cold-Adapted Microorganisms. The Class C Beta-Lactamase from the Antarctic Psychrophile Psychrobacter Immobilis A5

Feller, Georges; Zekhnini, Z.; Lamotte-Brasseur, Josette et al.

1997 • In European Journal of Biochemistry, 244 (1), p. 186-91

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https://hdl.handle.net/2268/16260

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10.1111/j.1432-1033.1997.00186.x

PubMed
9063463

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Abstract :

[en] A heat-labile beta-lactamase has been purified from culture supernatants of Psychrobacter immobilis A5 grown at 4 degrees C and the corresponding chromosomal ampC gene has been cloned and sequenced. All structural and kinetic properties clearly relate this enzyme to class C beta-lactamases. The kinetic parameters of P. immobilis beta-lactamase for the hydrolysis of some beta-lactam antibiotics are in the same range as the values recorded for the highly specialized cephalosporinases from pathogenic mesophilic bacteria. By contrast, the enzyme displays a low apparent optimum temperature of activity and a reduced thermal stability. Structural factors responsible for the latter property were analysed from the three-dimensional structure built by homology modelling. The deletion of proline residues in loops, the low number of arginine-mediated H-bonds and aromatic-aromatic interactions, the lower global hydrophobicity and the improved solvent interactions through additional surface acidic residues appear to be the main determinants of the enzyme flexibility.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Feller, Georges ; Université de Liège - ULiège > Département des sciences de la vie > Labo de biochimie

Zekhnini, Z.

Lamotte-Brasseur, Josette

Gerday, Charles ; Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)

Language :

English

Title :

Enzymes from Cold-Adapted Microorganisms. The Class C Beta-Lactamase from the Antarctic Psychrophile Psychrobacter Immobilis A5

Publication date :

10 February 1997

Journal title :

European Journal of Biochemistry

ISSN :

0014-2956

eISSN :

1432-1033

Publisher :

Blackwell, Oxford, United Kingdom

Volume :

244

Issue :

Pages :

186-91

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 26 January 2010

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