Article (Scientific journals)
Characterization of amylolysin, a novel lantibiotic from Bacillus amyloliquefaciens GA1
Arguelles Arias, Anthony; Ongena, Marc; Devreese, Bart et al.
2013In PLoS ONE, 8 (12)
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Keywords :
Bacillus amyloliquefaciens GA1; amylolysin; lantibiotic
Abstract :
[en] Background: Lantibiotics are heat-stable peptides characterized by the presence of thioether amino acid lanthionine and methyllanthionine. They are capable to inhibit the growth of Gram-positive bacteria, including Listeria monocytogenes, Staphylococcus aureus or Bacillus cereus, the causative agents of food-borne diseases or nosocomial infections. Lantibiotic biosynthetic machinery is encoded by gene cluster composed by a structural gene that codes for a pre-lantibiotic peptide and other genes involved in pre-lantibiotic modifications, regulation, export and immunity. Methodology/Findings: Bacillus amyloliquefaciens GA1 was found to produce an antimicrobial peptide, named amylolysin, active on an array of Gram-positive bacteria, including methicillin resistant S. aureus. Genome characterization led to the identification of a putative lantibiotic gene cluster that comprises a structural gene (amlA) and genes involved in modification (amlM), transport (amlT), regulation (amlKR) and immunity (amlFE). Disruption of amlA led to loss of biological activity, confirming thus that the identified gene cluster is related to amylolysin synthesis. MALDI-TOF and LC-MS analysis on purified amylolysin demonstrated that this latter corresponds to a novel lantibiotic not described to date. The ability of amylolysin to interact in vitro with the lipid II, the carrier of peptidoglycan monomers across the cytoplasmic membrane and the presence of a unique modification gene suggest that the identified peptide belongs to the group B lantibiotic. Amylolysin immunity seems to be driven by only two AmlF and AmlE proteins, which is uncommon within the Bacillus genus. Conclusion/Significance: Apart from mersacidin produced by Bacillus amyloliquefaciens strains Y2 and HIL Y-85,544728, reports on the synthesis of type B-lantibiotic in this species are scarce. This study reports on a genetic and structural characterization of another representative of the type B lantibiotic in B. amyloliquefaciens. Copyright: © 2013 Arguelles Arias et al.
Disciplines :
Microbiology
Author, co-author :
Arguelles Arias, Anthony ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Ongena, Marc ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Devreese, Bart
Terrak, Mohammed  ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Joris, Bernard ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
Fickers, Patrick ;  Université Libre de Bruxelles - ULB > Biotechnologies et Bioprocedes
Language :
English
Title :
Characterization of amylolysin, a novel lantibiotic from Bacillus amyloliquefaciens GA1
Publication date :
2013
Journal title :
PLoS ONE
eISSN :
1932-6203
Publisher :
Public Library of Science, San Franscisco, United States - California
Volume :
8
Issue :
12
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 29 January 2014

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