Article (Scientific journals)
Identification of type II and type III pyoverdine receptors from Pseudomonas aeruginosa.
de Chial, Magaly; Ghysels, Bart; Beatson, Scott A. et al.
2003In Microbiology, 149 (Pt 4), p. 821-31
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Keywords :
Amino Acid Sequence; Bacterial Outer Membrane Proteins/chemistry/genetics/metabolism; Culture Media; Cystic Fibrosis/microbiology; Ethylenediamines; Genetic Complementation Test; Humans; Iron/metabolism; Molecular Sequence Data; Mutation; Oligopeptides; Pigments, Biological/metabolism; Pseudomonas Infections/microbiology; Pseudomonas aeruginosa/drug effects/genetics/growth & development/metabolism; Pyocins/pharmacology; Receptors, Cell Surface/chemistry/genetics/metabolism; Sequence Analysis, DNA
Abstract :
[en] Pseudomonas aeruginosa produces, under conditions of iron limitation, a high-affinity siderophore, pyoverdine (PVD), which is recognized at the level of the outer membrane by a specific TonB-dependent receptor, FpvA. So far, for P. aeruginosa, three different PVDs, differing in their peptide chain, have been described (types I-III), but only the FpvA receptor for type I is known. Two PVD-producing P. aeruginosa strains, one type II and one type III, were mutagenized by a mini-TnphoA3 transposon. In each case, one mutant unable to grow in the presence of the strong iron chelator ethylenediaminedihydroxyphenylacetic acid (EDDHA) and the cognate PVD was selected. The first mutant, which had an insertion in the pvdE gene, upstream of fpvA, was unable to take up type II PVD and showed resistance to pyocin S3, which is known to use type II FpvA as receptor. The second mutant was unable to take up type III PVD and had the transposon insertion in fpvA. Cosmid libraries of the respective type II and type III PVD wild-type strains were constructed and screened for clones restoring the capacity to grow in the presence of PVD. From the respective complementing genomic fragments, type II and type III fpvA sequences were determined. When in trans, type II and type III fpvA restored PVD production, uptake, growth in the presence of EDDHA and, in the case of type II fpvA, pyocin S3 sensitivity. Complementation of fpvA mutants obtained by allelic exchange was achieved by the presence of cognate fpvA in trans. All three receptors posses an N-terminal extension of about 70 amino acids, similar to FecA of Escherichia coli, but only FpvAI has a TAT export sequence at its N-terminal end.
Disciplines :
Microbiology
Biochemistry, biophysics & molecular biology
Author, co-author :
de Chial, Magaly 
Ghysels, Bart  ;  Vrije Universiteit Brussel - VUB > Laboratory of Microbial Interactions
Beatson, Scott A.
Geoffroy, Valerie
Meyer, Jean Marie
Pattery, Theresa
Baysse, Christine
Chablain, Patrice
Parsons, Yasmin N.
Winstanley, Craig
Cordwell, Stuart J.
Cornelis, Pierre
 These authors have contributed equally to this work.
Language :
English
Title :
Identification of type II and type III pyoverdine receptors from Pseudomonas aeruginosa.
Publication date :
2003
Journal title :
Microbiology
ISSN :
1350-0872
eISSN :
1465-2080
Publisher :
Society for General Microbiology, United Kingdom
Volume :
149
Issue :
Pt 4
Pages :
821-31
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 29 January 2014

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