Characterization of a novel IMP-28 metallo-β-lactamase from a Spanish Klebsiella oxytoca clinical isolate

[en] An isolate of Klebsiella oxytoca carrying a novel IMP metallo-β-lactamase was discovered in Madrid, Spain. The bla(IMP-28) gene is part of a chromosomally located class I integron. The IMP-28 k(cat)/K(m) values for ampicillin, ceftazidime, and cefepime and, to a lesser extent, imipenem and meropenem, are clearly lower than those of IMP-1. The His306Gln mutation may induce important modifications of the L3 loop and thus of substrate accessibility and hydrolysis and be the main reason for this behavior.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Pérez-Llarena, FJ

Fernández, A

Zamorano, L

Kerff, Frédéric ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Beceiro, A

Aracil, B

Cercenado, E

Miro, E

Oliver, A

Oteo, J

Navarro, F

Bou, G

Language :

English

Title :

Characterization of a novel IMP-28 metallo-β-lactamase from a Spanish Klebsiella oxytoca clinical isolate

Publication date :

August 2012

Journal title :

Antimicrobial Agents and Chemotherapy

ISSN :

0066-4804

eISSN :

1098-6596

Publisher :

American Society for Microbiology (ASM), Washington, United States - District of Columbia

Volume :

Issue :

Pages :

4540-3

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 20 January 2014

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Bibliography

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