Reference : Reprogramming of fatty acid and oxylipin synthesis in rhizobacteria-induced systemic ...
Scientific journals : Article
Life sciences : Biotechnology
Physical, chemical, mathematical & earth Sciences : Chemistry
Life sciences : Food science
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
http://hdl.handle.net/2268/160239
Reprogramming of fatty acid and oxylipin synthesis in rhizobacteria-induced systemic resistance in tomato
English
Mariutto, Martin [Université de Liège - ULiège > Life Sciences > Laboratory of Plant Molecular Biology and Biotechnology > >]
Fauconnier, Marie-Laure mailto [Université de Liège - ULiège > Chimie et bio-industries > Chimie générale et organique >]
Ongena, Marc mailto [Université de Liège - ULiège > Chimie et bio-industries > Bio-industries >]
Laloux, Morgan []
Wathelet, Jean-Paul mailto [Université de Liège - ULiège > Chimie et bio-industries > Chimie générale et organique >]
du Jardin, Patrick mailto [Université de Liège - ULiège > Sciences agronomiques > Biologie végétale >]
Thonart, Philippe mailto [Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Dommes, Jacques mailto [Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales >]
2014
Plant Molecular Biology
Kluwer Academic Publishers
84
4-5
455-476
Yes (verified by ORBi)
International
0167-4412
1573-5028
Dordrecht
The Netherlands
[en] Induced systemic resistance ; Biocontrol ; Plant growth-promoting rhizobacteria ; Oxylipin ; Fatty acid ; Plant defense
[en] The rhizobacterium Pseudomonas putida BTP1 stimulates induced systemic resistance (ISR) in tomato. A previous work showed that the resistance is associated in leaves with the induction of the first enzyme of the oxylipin pathway, the lipoxygenase (LOX), leading to a faster accumulation of its product, the free 13-hydroperoxy octadecatrienoic acid (13-HPOT), 2 days after Botrytis cinerea inoculation. In the present study, we further investigated the stimulation of the oxylipin pathway: metabolites and enzymes of the pathway were analyzed to understand the fate of the 13-HPOT in ISR. Actually the stimulation began upstream the LOX: free linolenic acid accumulated faster in P. putida BTP1-treated plants than in control. Downstream, the LOX products 13-fatty acid hydroperoxides esterified to galactolipids and phospholipids were more abundant in bacterized plants than in control before infection. These metabolites could constitute a pool that will be used after pathogen attack to produce free fungitoxic metabolites through the action of phospholipase A2, which is enhanced in bacterized plants upon infection. Enzymatic branches which can use as substrate the fatty acid hydroperoxides were differentially regulated in bacterized plants in comparison to control plants, so as to lead to the accumulation of the most fungitoxic compounds against B. cinerea. Our study, which is the first to demonstrate the accumulation of an esterified defense metabolite during rhizobacteria-mediated induced systemic resistance, showed that the oxylipin pathway is differentially regulated. It suggests that this allows the plant to prepare to a future infection, and to respond faster and in a more effective way to B. cinerea invasion.
Researchers
http://hdl.handle.net/2268/160239
10.1007/s11103-013-0144-y
http://download.springer.com/static/pdf/600/art%253A10.1007%252Fs11103-013-0144-y.pdf?auth66=1384006184_2601dd070e2ff4c83a93eb415746fb5d&ext=.pdf
The final publication is available at link.springer.com.
The online version of this article (doi:10.1007/s11103-013-0144-y) contains supplementary material, which is available to authorized users.

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