Article (Scientific journals)
Reprogramming of fatty acid and oxylipin synthesis in rhizobacteria-induced systemic resistance in tomato
Mariutto, Martin; Fauconnier, Marie-Laure; Ongena, Marc et al.
2014In Plant Molecular Biology, 84 (4-5), p. 455-476
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Keywords :
Induced systemic resistance; Biocontrol; Plant growth-promoting rhizobacteria; Oxylipin; Fatty acid; Plant defense
Abstract :
[en] The rhizobacterium Pseudomonas putida BTP1 stimulates induced systemic resistance (ISR) in tomato. A previous work showed that the resistance is associated in leaves with the induction of the first enzyme of the oxylipin pathway, the lipoxygenase (LOX), leading to a faster accumulation of its product, the free 13-hydroperoxy octadecatrienoic acid (13-HPOT), 2 days after Botrytis cinerea inoculation. In the present study, we further investigated the stimulation of the oxylipin pathway: metabolites and enzymes of the pathway were analyzed to understand the fate of the 13-HPOT in ISR. Actually the stimulation began upstream the LOX: free linolenic acid accumulated faster in P. putida BTP1-treated plants than in control. Downstream, the LOX products 13-fatty acid hydroperoxides esterified to galactolipids and phospholipids were more abundant in bacterized plants than in control before infection. These metabolites could constitute a pool that will be used after pathogen attack to produce free fungitoxic metabolites through the action of phospholipase A2, which is enhanced in bacterized plants upon infection. Enzymatic branches which can use as substrate the fatty acid hydroperoxides were differentially regulated in bacterized plants in comparison to control plants, so as to lead to the accumulation of the most fungitoxic compounds against B. cinerea. Our study, which is the first to demonstrate the accumulation of an esterified defense metabolite during rhizobacteria-mediated induced systemic resistance, showed that the oxylipin pathway is differentially regulated. It suggests that this allows the plant to prepare to a future infection, and to respond faster and in a more effective way to B. cinerea invasion.
Disciplines :
Chemistry
Food science
Biotechnology
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Mariutto, Martin;  Université de Liège - ULiège > Life Sciences > Laboratory of Plant Molecular Biology and Biotechnology
Fauconnier, Marie-Laure  ;  Université de Liège - ULiège > Chimie et bio-industries > Chimie générale et organique
Ongena, Marc ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Laloux, Morgan
Wathelet, Jean-Paul ;  Université de Liège - ULiège > Chimie et bio-industries > Chimie générale et organique
du Jardin, Patrick  ;  Université de Liège - ULiège > Sciences agronomiques > Biologie végétale
Thonart, Philippe ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Dommes, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales
Language :
English
Title :
Reprogramming of fatty acid and oxylipin synthesis in rhizobacteria-induced systemic resistance in tomato
Publication date :
2014
Journal title :
Plant Molecular Biology
ISSN :
0167-4412
eISSN :
1573-5028
Publisher :
Kluwer Academic Publishers, Dordrecht, Netherlands
Volume :
84
Issue :
4-5
Pages :
455-476
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 December 2013

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