A colorimetric method for the determination of lipase activity in soil

Margesin, R.; Feller, Georges; Hammerle, M.; Stegner, U.; Schinner, F.

doi:10.1023/A:1013801131553

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Article (Scientific journals)

A colorimetric method for the determination of lipase activity in soil

Margesin, R.; Feller, Georges; Hammerle, M. et al.

2002 • In Biotechnology Letters, 24 (1), p. 27-33

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/16013

DOI
10.1023/A:1013801131553

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Keywords :

lipase activity; mineral-oil-contamination; p-nitrophenyl butyrate; soil

Abstract :

[en] A colorimetric method for the determination of lipase activity in soil has been developed. Using p-nitrophenyl butyrate as substrate, soil samples are incubated at 30 degreesC and pH 7.25 for 10 min. After cooling on ice and centrifugation, the released p-nitrophenol is determined at 400 nm. To allow for the adsorption of p-nitrophenol onto soil, a calibration curve is prepared in the presence of soil.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Margesin, R.

Feller, Georges ; Université de Liège - ULiège > Département des sciences de la vie > Labo de biochimie

Hammerle, M.

Stegner, U.

Schinner, F.

Language :

English

Title :

A colorimetric method for the determination of lipase activity in soil

Publication date :

January 2002

Journal title :

Biotechnology Letters

ISSN :

0141-5492

eISSN :

1573-6776

Publisher :

Kluwer Academic Publ, Dordrecht, Netherlands

Volume :

Issue :

Pages :

27-33

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 26 January 2010

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Bibliography

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