Master’s dissertation (Dissertations and theses)
Contribution à l’étude des peptides obliques impliqués dans des transconformations
Crowet, Jean-Marc
2003
 

Files


Full Text
TFE_JMC.pdf
Author postprint (1.22 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] The study of the amyloidogenic proteins is important to understand the transconformational processes. These proteins have a unique common feature : they undergo a conformational change leading to the formation of highly structured fibrils that are mainly β conformed. These proteins are difficult to study experimentally due to their low solubility and their tendency to aggregate rapidly. Recently, tilted peptides have been detected in such proteins. These peptides are short fragments (11 to 18 residues) that adopt a tilted orientation when they interact with a hydrophilic/hydrophobic interface. This property is due to the presence of an asymmetric hydrophobicity gradient when they are helical. Those peptides are able to destabilize organized molecular systems. Amyloidogenic proteins containing such peptides are the PrP protein, the Aȕ peptide, and the bacterial prions Ure2p and Sup35p. The presence of such fragments in transconformational proteins has led to the hypothesis that tilted peptides could be implied in the conformational change of amyloidogenic proteins. These peptides could play a role through their destabilizing properties and/or their structural lability. The aim of this work was to detect tilted peptides among other amyloidogenic proteins by molecular modelling. One of the detected peptides is tested experimentally to evidence its lipid destabilizing properties. Five tilted peptides from four different proteins have been detected among 27 amyloidogenic proteins. The tilted fragment from the α-synuclein sequence, a protein involved in Parkinson disease, has been synthetized and tested for lipid fusion. The fusogenic assays pointed out its destabilizing properties. These results support the hypothesis that tilted peptides could be involved in transconformational processes.
Research center :
Centre de Biophysique Moléculaire Numérique
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Crowet, Jean-Marc ;  Université de Liège - ULiège > Chimie et bio-industries > Biophysique moléc. numér.
Language :
French
Title :
Contribution à l’étude des peptides obliques impliqués dans des transconformations
Alternative titles :
[en] Contribution to the Study of Tilted Peptides Involved in Protein Transconforamtion
Defense date :
11 September 2003
Number of pages :
86
Institution :
ULiège. GxABT - Liège Université. Gembloux Agro-Bio Tech
Degree :
Ingénieur Chimiste et des Bio-Industries
Promotor :
Brasseur, Robert ;  Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
Lins, Laurence  ;  Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
Paquot, Michel ;  Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies
President :
Portetelle, Daniel ;  Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies
Jury member :
Deroanne, Claude
Name of the research project :
Etude de l’implication des peptides obliques dans les phénomènes de transconformation
Available on ORBi :
since 10 December 2013

Statistics


Number of views
18 (4 by ULiège)
Number of downloads
834 (15 by ULiège)

Bibliography


Similar publications



Contact ORBi