Effect of Inducers and Pcbs on the Cytochrome P450 Enzymes in Cultured Quail Hepatocytes

[en] Hepatocytes isolated from fetal quail livers (Coturnix coturnix japonica) were cultured in vitro. Their capacity to metabolize drugs and xenobiotics was explored with typical cytochrome P450 substrates: ethoxycoumarin (known to be metabolized by several P450s), ethoxyresorufin (essentially dealkylated by P450IA1), and testosterone (specifically hydroxylated at several positions by several P450s). The cells could be kept metabolically active in culture for at least 4 days. Their drug-metabolizing activities were inducible by the usual P450 inducers, like phenobarbital and benzanthracene, but also by Aroclor 1254, a PCB mixture. The results obtained indicate that this experimental model could certainly be very helpful in ecotoxicological studies.

Disciplines :

Pharmacy, pharmacology & toxicology

Author, co-author :

Roelandt, L.

Dubois, Maryline

Todaro, Anna

Thomé, Jean-Pierre ; Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Ecologie animale et écotoxicologie

Kremers, Pierre

Language :

English

Title :

Effect of Inducers and Pcbs on the Cytochrome P450 Enzymes in Cultured Quail Hepatocytes

Publication date :

July 1995

Journal title :

Ecotoxicology and Environmental Safety

ISSN :

0147-6513

eISSN :

1090-2414

Publisher :

Elsevier, Atlanta, United States - Florida

Volume :

Issue :

Pages :

158-63

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

SPPS impulse program "Health Hazards HH/12/46

Funders :

grant from the Belgian Ministery of Research and Development

Available on ORBi :

since 27 November 2013

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