Article (Scientific journals)
Ectopic bone formation by 3D porous calcium phosphate-Ti6Al4V hybrids produced by perfusion electrodeposition.
Chai, Yoke Chin; Kerckhofs, Greet; Roberts, Scott J. et al.
2012In Biomaterials, 33 (16), p. 4044-58
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Keywords :
Base Sequence; Bone Development; Calcium Phosphates/chemistry; Cell Differentiation; Cell Proliferation; Cells, Cultured; DNA Primers; Down-Regulation; Electrochemical Techniques; Humans; Microscopy, Electron, Scanning; Osteoprotegerin/genetics; Polymerase Chain Reaction; Solubility; Surface Properties; Titanium/chemistry; Tomography, X-Ray Computed
Abstract :
[en] Successful clinical repair of non-healing skeletal defects requires the use of bone substitutes with robust bone inductivity and excellent biomechanical stability. Thus, three-dimensionally functionalised porous calcium phosphate-Ti6Al4V (CaP-Ti) hybrids were produced by perfusion electrodeposition, and the in vitro and in vivo biological performances were evaluated using human periosteum derived cells (hPDCs). By applying various current densities at the optimised deposition conditions, CaP coatings with sub-micrometer to nano-scale porous crystalline structures and different ion dissolution kinetics were deposited on the porous Ti6Al4V scaffolds. These distinctive physicochemical properties caused a significant impact on in vitro proliferation, osteogenic differentiation, and matrix mineralisation of hPDCs. This includes a potential role of hPDCs in mediating osteoclastogenesis for the resorption of CaP coatings, as indicated by a significant down-regulation of osteoprotegerin (OPG) gene expression and by the histological observation of abundant multi-nucleated giant cells near to the coatings. By subcutaneous implantation, the produced hybrids induced ectopic bone formation, which was highly dependent on the physicochemical properties of the CaP coating (including the Ca(2+) dissolution kinetics and coating surface topography), in a cell density-dependent manner. This study provided further insight on stem cell-CaP biomaterial interactions, and the feasibility to produced bone reparative units that are predictively osteoinductive in vivo by perfusion electrodeposition technology.
Disciplines :
Engineering, computing & technology: Multidisciplinary, general & others
Author, co-author :
Chai, Yoke Chin
Kerckhofs, Greet ;  Université de Liège - ULiège > Département d'aérospatiale et mécanique > Génie biomécanique
Roberts, Scott J.
Van Bael, Simon
Schepers, Evert
Vleugels, Jozef
Luyten, Frank P.
Schrooten, Jan
Language :
English
Title :
Ectopic bone formation by 3D porous calcium phosphate-Ti6Al4V hybrids produced by perfusion electrodeposition.
Publication date :
2012
Journal title :
Biomaterials
ISSN :
0142-9612
eISSN :
1878-5905
Publisher :
Elsevier, United Kingdom
Volume :
33
Issue :
16
Pages :
4044-58
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright A(c) 2012 Elsevier Ltd. All rights reserved.
Available on ORBi :
since 14 November 2013

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