The effect of pore geometry on the in vitro biological behavior of human periosteum-derived cells seeded on selective laser-melted Ti6Al4V bone scaffolds.
[en] The specific aim of this study was to gain insight into the influence of scaffold pore size, pore shape and permeability on the in vitro proliferation and differentiation of three-dimensional (3-D) human periosteum-derived cell (hPDC) cultures. Selective laser melting (SLM) was used to produce six distinct designed geometries of Ti6Al4V scaffolds in three different pore shapes (triangular, hexagonal and rectangular) and two different pore sizes (500 mum and 1000 mum). All scaffolds were characterized by means of two-dimensional optical microscopy, 3-D microfocus X-ray computed tomography (micro-CT) image analysis, mechanical compression testing and computational fluid dynamical analysis. The results showed that SLM was capable of producing Ti6Al4V scaffolds with a broad range of morphological and mechanical properties. The in vitro study showed that scaffolds with a lower permeability gave rise to a significantly higher number of cells attached to the scaffolds after seeding. Qualitative analysis by means of live/dead staining and scanning electron micrography showed a circular cell growth pattern which was independent of the pore size and shape. This resulted in pore occlusion which was found to be the highest on scaffolds with 500 mum hexagonal pores. Interestingly, pore size but not pore shape was found to significantly influence the growth of hPDC on the scaffolds, whereas the differentiation of hPDC was dependent on both pore shape and pore size. The results showed that, for SLM-produced Ti6Al4V scaffolds with specific morphological and mechanical properties, a functional graded scaffold will contribute to enhanced cell seeding and at the same time can maintain nutrient transport throughout the whole scaffold during in vitro culturing by avoiding pore occlusion.
Disciplines :
Engineering, computing & technology: Multidisciplinary, general & others
Author, co-author :
Van Bael, S.
Chai, Y. C.
Truscello, S.
Moesen, M.
Kerckhofs, Greet ; Université de Liège - ULiège > Département d'aérospatiale et mécanique > Génie biomécanique
Van Oosterwyck, H.
Kruth, J.-P.
Schrooten, J.
Language :
English
Title :
The effect of pore geometry on the in vitro biological behavior of human periosteum-derived cells seeded on selective laser-melted Ti6Al4V bone scaffolds.
Publication date :
2012
Journal title :
Acta Biomaterialia
ISSN :
1742-7061
eISSN :
1878-7568
Publisher :
Elsevier, Netherlands
Volume :
8
Issue :
7
Pages :
2824-34
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
D.W. Hutmacher Scaffolds in tissue engineering bone and cartilage Biomaterials 21 2000 2529 2543
D.W. Hutmacher, J.T. Schantz, C.X.F. Lam, K.C. Tan, and T.C. Lim State of the art and future directions of scaffold-based bone engineering from a biomaterials perspective 2007 John Wiley Chichester pp. 245-60
S.J. Hollister Scaffold design and manufacturing: from concept to clinic 2009 Wiley-VCH Hoboken, NJ pp. 3330-42
S.J. Hollister Porous scaffold design for tissue engineering Nat Mater 4 2005 518 524
K. Alvarez, and H. Nakajima Metallic scaffolds for bone regeneration. Materials 2 2009 790 832
E. Sachlos, and J.T. Czernuszka Making tissue engineering scaffolds work. Review on the application of solid freeform fabrication technology to the production of tissue engineering scaffolds Eur Cells Mater 5 2003 29 40
L.E. Murr, S.M. Gaytan, F. Medina, E. Martinez, J.L. Martinez, and D.H. Hernandez Characterization of Ti-6Al-4V open cellular foams fabricated by additive manufacturing using electron beam melting Mater Sci Eng A 527 2009 1861 1868
L. Mullen, R.C. Stamp, P. Fox, E. Jones, C. Ngo, and C.J. Sutcliffe Selective laser melting: a unit cell approach for the manufacture of porous, titanium, bone in-growth constructs, suitable for orthopedic applications. II. Randomized structures J Biomed Mater Res Part B: Appl Biomater 98 2010 178 188
J.M. Sobral, S.G. Caridade, R.A. Sousa, J.F. Mano, and R.L. Reis Three-dimensional plotted scaffolds with controlled pore size gradients: effect of scaffold geometry on mechanical performance and cell seeding efficiency Acta Biomater 7 2011 1009 1018
S. Van Bael, G. Kerckhofs, M. Moesen, G. Pyka, J. Schrooten, and J.P. Kruth Micro-CT-based improvement of geometrical and mechanical controllability of selective laser melted Ti6Al4V porous structures Mater Sci Eng A 528 2011 7423 7431
A. Fukuda, M. Takemoto, T. Saito, S. Fujibayashi, M. Neo, and D.K. Pattanayak Osteoinduction of porous Ti implants with a channel structure fabricated by selective laser melting Acta Biomater 7 2011 2327 2336
K.F. Leong, C.M. Cheah, and C.K. Chua Solid freeform fabrication of three-dimensional scaffolds for engineering replacement tissues and organs Biomaterials 24 2003 2363 2378
M. Rumpler, A. Woesz, J.W. Dunlop, J.T. van Dongen, and P. Fratzl The effect of geometry on three-dimensional tissue growth J R Soc Interface 5 2008 1173 1180
P. Warnke, T. Douglas, P. Wollny, E. Sherry, M. Steiner, and S. Galonska Rapid prototyping: porous titanium alloy scaffolds produced by selective laser melting for bone tissue engineering Tissue Eng Part C: Methods 15 2009 115 124
D.A. Hollander, M. von Walter, T. Wirtz, R. Sellei, B. Schmidt-Rohlfing, and O. Paar Structural, mechanical and in vitro characterization of individually structured Ti-6Al-4V produced by direct laser forming Biomaterials 27 2006 955 963
S. Van Bael, B. Vandenbroucke, G. Kerckhofs, J. Schrooten, and J. Kruth Design and production of bone scaffolds with selective laser melting 2009 38th TMS Annual Meeting and Exhibition TMS San Francisco, CA
S. Van Bael, G. Kerckhofs, M. Moesen, G. Pyka, J. Schrooten, and J.P. Kruth Micro-CT-based improvement of geometrical and mechanical controllability of selective laser melted Ti6Al4V porous structures Mater Sci Eng A 528 2011 7423 7431
J. Van Vaerenbergh Process optimisation in selective laser melting 2008 Enschede Twente
Y.C. Chai, S.J. Roberts, J. Schrooten, and F.P. Luyten Probing the osteoinductive effect of calcium phosphate by using an in vitro biomimetic model Tissue Eng Part A 17 2011 1083 1097
T. Hildebrand, and P. Rüegsegger A new method for the model-independent assessment of thickness in three-dimensional images J Microsc 185 1997 67 75
Kerckhofs G. Morphological and mechanical quantification of porous structures by means of micro-CT, doctoral thesis, K.U. Leuven, 2009.
G. Kerckhofs, J. Schrooten, T. Van Cleynenbreugel, S.V. Lomov, and M. Wevers Validation of X-ray microfocus computed tomography as an imaging tool for porous structures Rev Sci Instrum 79 2008 013711 13719
T. Craeghs, S. Clijsters, E. Yasa, F. Bechmann, S. Berumen, and J. Kruth Determination of geometrical factors in layerwise laser melting using optical process monitoring Opt Lasers Eng 49 2011 1440 1446
V. Karageorgiou, and D. Kaplan Porosity of 3-D biomaterial scaffolds and osteogenesis Biomaterials 26 2005 5474 5491
J.H. Scott Scaffold engineering: a bridge to where? Biofabrication 1 2009 012001
M.J. Moore, E. Jabbari, E.L. Ritman, L. Lu, B.L. Currier, and A.J. Windebank Quantitative analysis of interconnectivity of porous biodegradable scaffolds with micro-computed tomography J Biomed Mater Res, Part A 71A 2004 258 267
S.T. Ho, and D.W. Hutmacher A comparison of micro CT with other techniques used in the characterization of scaffolds Biomaterials 27 2006 1362 1376
O. Ersoy, E. Sen, E. Aydar, I. Tatar, and H.H. Çelik Surface area and volume measurements of volcanic ash particles using micro-computed tomography (micro-CT): a comparison with scanning electron microscope (SEM) stereoscopic imaging and geometric considerations J Volcanol Geoth Res 196 2010 281 286
S. Truscello, G. Kerckhofs, S. Van Bael, G. Pyka, J. Schrooten, and H. Van Oosterwyck Prediction of permeability of regular scaffolds for skeletal tissue engineering: a combined computational and experimental study Acta Biomater 8 2012 1648 1658
J. Parthasarathy, B. Starly, S. Raman, and A. Christensen Mechanical evaluation of porous titanium (Ti6Al4V) structures with electron beam melting (EBM) J Mech Behav Biomed Mater 3 2010 249 259
X. Li, C. Wang, W. Zhang, and Y. Li Fabrication and characterization of porous Ti6Al4V parts for biomedical applications using electron beam melting process Mater Lett 63 2009 403 405
Carter DR, WC H. The compressive behavior of bone as a two-phase porous structure. J Bone and Joint Surg 1977;59:954-62.
J. Peterson, and P.C. Dechow Material properties of the human cranial vault and zygoma Anat Rec Part A 274A 2003 785 797
S.A. Goldstein The mechanical properties of trabecular bone dependence on the anatomuc location and function J Biomech 20 1987 1055 1061
Saartje. Impens, Yantian. Chen, Steven. Mullens, F. Luyten, and J. Schrooten Controlled cell-seeding methodologies: a first step toward clinically relevant bone tissue engineering strategies Tissue Eng Part C: Methods 16 2010 1575 1583
L. Shor, X. Wen, M. Gandhi, and W. Sun Fabrication of three-dimensional polycaprolactone/hydroxyapatite tissue scaffolds and osteoblast-scaffold interactions in vitro Biomaterials 28 2007 5291 5297
L.H. Li, K.P. Kommareddy, C. Pilz, C.R. Zhou, P. Fratzl, and I. Manjubala In vitro bioactivity of bioresorbable porous polymeric scaffolds incorporating hydroxyapatite microspheres Acta Biomater 6 2010 2525 2531
P. Fratzl, K.P. Kommareddy, C. Lange, M. Rumpler, J.W.C. Dunlop, and I. Manjubala Two stages in three-dimensional in vitro growth of tissue generated by osteoblastlike cells Biointerphases 5 2010 45 52
W. Xue, B.V. Krishna, A. Bandyopadhyay, and S. Bose Processing and biocompatibility evaluation of laser processed porous titanium Acta Biomater 3 2007 1007 1018
V.K. Balla, S. Bodhak, S. Bose, and A. Bandyopadhyay Porous tantalum structures for bone implants: fabrication, mechanical and in vitro biological properties Acta Biomater 6 2010 3349 3359
F.P.W. Melchels, A.M.C. Barradas, C.A. van Blitterswijk, J. de Boer, J. Feijen, and D.W. Grijpma Effects of the architecture of tissue engineering scaffolds on cell seeding and culturing Acta Biomater 6 2010 4208 4217
E.G. Khaled, M. Saleh, S. Hindocha, M. Griffin, and W.S. Khan Tissue engineering for bone production - stem cells, gene therapy and scaffolds Open Orthop J 5 Suppl 2 2011 289 295
G. Brown, P.J. Hughes, and R.H. Michell Cell differentiation and proliferation - simultaneous but independent? Exp Cell Res 291 2003 282 288
S.J. Roberts, Y. Chen, M. Moesen, J. Schrooten, and F.P. Luyten Enhancement of osteogenic gene expression for the differentiation of human periosteal derived cells Stem Cell Res 7 2011 137 144
B. Dabrowski, W. Swieszkowski, D. Godlinski, and K.J. Kurzydlowski Highly porous titanium scaffolds for orthopaedic applications J Biomed Mater Res B: Appl Biomater 95B 2010 53 61
G. Ryan, A. Pandit, and D.P. Apatsidis Fabrication methods of porous metals for use in orthopaedic applications Biomaterials 27 2006 2651 2670