Reference : Horizontal gene transfer from Eukarya to Bacteria and domain shuffling: the alpha-amy...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Horizontal gene transfer from Eukarya to Bacteria and domain shuffling: the alpha-amylase model
Da Lage, J. L. [> > > >]
Feller, Georges mailto [Université de Liège - ULiège > Département des sciences de la vie > Labo de biochimie >]
Janecek, S. [> > > >]
Cellular and Molecular Life Sciences
Yes (verified by ORBi)
[en] horizontal gene transfer ; alpha-amylase ; C-terminal domain ; Microbulbifer degradans
[en] alpha-Amylases are present in all kingdoms of the living world. Despite strong conservation of the tertiary structure, only a few amino acids are conserved in interkingdom comparisons. Animal alpha-amylases are characterized by several typical motifs and biochemical properties. A few cases of such alpha-amylases have been previously reported in some eubacterial species. We screened the bacterial genomes available in the sequence databases for new occurrences of animal-like alpha-amylases. Three novel cases were found, which belong to unrelated bacterial phyla: Chloroflexus aurantiacus, Microbulbifer degradans, and Thermobifida fusca. All the animal-like alpha-amylases in Bacteria probably result from repeated horizontal gene transfer from animals. The M. degradans genome also contains bacterial-type and plant-type alpha-amylases in addition to the animal-type one. Thus, this species exhibits alpha-amylases of animal, plant, and bacterial origins. Moreover, the similarities in the extra C-terminal domains (different from both the alpha-amylase domain C and the starch-binding domain), when present, also suggest interkingdom as well as intragenomic shuffling.

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