Unpublished conference/Abstract (Scientific congresses and symposiums)
Study of the virulence of serotypes 4 and 9 of the Orbivirus African horse sickness virus in two mouse models
De la Grandière de Noronha Cotta, Maria Ana; Zonta, William; Mauroy, Axel et al.
20135th European Congress of Virology
 

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Keywords :
Orbivirus; African horse sickness virus; animal model
Abstract :
[en] African horse sickness (AHSV) is an infectious disease caused by a double stranded positive RNA virus which belongs to the family Reoviridae, genus Orbivirus. The virus has nine serotypes and is transmitted by a culicoides biting midge, principally Culicoides imicola. The establishment of an experimental model is needed for the investigation of the pathogenesis of this infection. Two mouse models, interferon-α receptor knock-out mice (A129 KO or IFNAR -/-) and immunocompetent mice (A129 WT), were tested. The viruses used for mice inoculations belonged to the two serotypes which caused epidemics in Europe, serotypes 4 and 9. The virus was inoculated by subcutaneous (SC) route and/or by intra-nasal (IN) route. Whole blood samples were taken from each mouse at regular intervals. The organs (liver, spleen, kidney, lung and brain) were taken at the end of the experiment or when the most affected mice were euthanized. All these samples were tested by a qRT-PCR targeting AHSV genome segment 7. Both serotypes of AHSV were detected by qRT-PCR until three weeks post-infection in blood of IFNAR -/- mice and A129 WT mice infected by SC route. Serotype 4 shows a higher peak of viremia than serotype 9. The peak of viremia was measured between day 2 and day 4 post-infection. These results demonstrate the potential of the immunodeficient mouse model for both clinical and biological features. The setting up of this mouse model has developed a tool for efficient in vivo study of AHSV. Research supported by the Belgium Federal Public Service, Health, Food Chain Safety and Environment.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
De la Grandière de Noronha Cotta, Maria Ana ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie vétérinaire et maladies virales animales
Zonta, William ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie vétérinaire et maladies virales animales
Mauroy, Axel ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie vétérinaire et maladies virales animales
Dal Pozzo, Fabiana ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Epidémiologie et analyse des risques appl. aux sc. vétér.
Thiry, Etienne ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie vétérinaire et maladies virales animales
Language :
English
Title :
Study of the virulence of serotypes 4 and 9 of the Orbivirus African horse sickness virus in two mouse models
Publication date :
12 September 2013
Event name :
5th European Congress of Virology
Event organizer :
European Society for Virology
Event place :
Lyon, France
Event date :
du 11 septembre 2013 au 14 septembre 2013
Audience :
International
Name of the research project :
INDEVIREQ 2.0
Funders :
SPF Santé - Service Public Fédéral Santé publique. Sécurité de la Chaîne alimentaire et Environnement [BE]
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since 07 October 2013

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