Article (Scientific journals)
Determination of inhibitory potency of argatroban toward thrombin by electrophoretically mediated microanalysis
Pochet, Lionel; Servais, Anne-Catherine; Farcas, Elena et al.
2013In Talanta, 116, p. 719-725
Peer Reviewed verified by ORBi
 

Files


Full Text
Pochet Talanta 2013.pdf
Publisher postprint (654.76 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
EMMA; Thrombin; Argatroban; Enzymatic assay
Abstract :
[en] Developing an EMMA method for enzymatic assay remains a challenge, particularly using UV detection. Indeed, it is necessary to optimize the separation conditions while allowing the enzymatic reaction to occur within the capillary respecting kinetic constraints and achieving enough sensitivity. In this work, such EMMA methodology was set up to evaluate the inhibitory potency of drugs toward thrombin, a serine protease implicated in the coagulation cascade. To achieve our goal, the separation buffer, the injection sequence, the internal standard and the chromogenic substrate were investigated. The newly developed system was then assessed determining the kinetic Km constant for the selected substrate and compared with the results obtained with a continuous spectrophotometer cell assay. Secondly, the Ki inhibitory constant of the thrombin inhibitor argatroban was determined and found in agreement with the published value.
Research center :
CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Pochet, Lionel ;  Facultés Universitaires Notre-Dame de la Paix - Namur - FUNDP > Namur Medicine & Drug Innovation Center (NaMEDIC - Narilis)
Servais, Anne-Catherine   ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Farcas, Elena ;  Université de Liège - ULiège
Bettonville, Virginie ;  Université de Liège - ULiège
Bouckaert, Charlotte;  Facultés Universitaires Notre-Dame de la Paix - Namur - FUNDP > Namur Medicine & Drug Innovation Center (NaMEDIC - Narilis)
Fillet, Marianne ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
 These authors have contributed equally to this work.
Language :
English
Title :
Determination of inhibitory potency of argatroban toward thrombin by electrophoretically mediated microanalysis
Publication date :
2013
Journal title :
Talanta
ISSN :
0039-9140
eISSN :
1873-3573
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
116
Pages :
719-725
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
ULiège FSR - Université de Liège. Fonds spéciaux pour la recherche [BE]
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Fonds Léon Fredericq [BE]
Available on ORBi :
since 28 August 2013

Statistics


Number of views
76 (22 by ULiège)
Number of downloads
3 (3 by ULiège)

Scopus citations®
 
28
Scopus citations®
without self-citations
21
OpenCitations
 
28

Bibliography


Similar publications



Contact ORBi