[en] Different parameters as temperature, pressure, solvent mass and sample granulometry governing the extraction yield of tagitinin C from the aerial parts of Tithonia diversifolia were optimised. An experimental design was carried out to map the effects of pressure (at 20.3, 30.4 and 40.5 MPa) and temperature (at 40, 60 and 80degreesC) on the extraction yield of the active component and to determine the optimal conditions for the extraction of tagitinin C from T diversifolia. The best conditions are met for a pressure of 35.0 MPa and a temperature of 68degreesC. The effect of the particle size was studied under low pressure (13.7 MPa) and temperature (40degreesC) conditions, which failed to extract quantitatively the tagitinin C from leaves sieved to 250 mum size. The reduction of the particle size increased the extraction yield which became comparable to that of the optimised SFE for the particle in the range of 0-63 mum. From the analysis of extraction kinetic curves of 200 mg of plant with supercritical carbon dioxide (range of 5-30 g), it appears that 15 g of this supercritical fluid is never limiting. The optimised supercritical fluid extraction (SFE) was compared favourably to Soxhlet extraction with dichloromethane (S) and to maceration followed by lixiviation with diethyl ether (ML), which gave similar extraction yields but higher extract content of tagitinin C were found using SFE (15.6 and 30.7% w/w tagitinin C in S and ML extracts, respectively, versus 52.8% in SFE extract).
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Ziemons, Eric ; Université de Liège - ULiège > Chimie analytique
Goffin, Eric ; Université de Liège - ULiège > Chimie pharmaceutique
Lejeune, Robert ; Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Proença da Cunha, A. P.
Angenot, Luc ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Thunus, Léopold ; Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Language :
English
Title :
Supercritical carbon dioxide extraction of tagitinin C from Tithonia diversifolia
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Bibliography
Goffin E., Ziemons E., De Mol P., de Ceu de Madureira M., Martins A.P., Proenca da Cunha A., Philippe G., Tits M., Angenot L., Frederich M. In vitro antiplasmodial activity of Tithonia diversifolia and identification of its main active constituent: tagitinin C. Planta Med. 68:2002;543
Pal R., Kulshreshtha D.K., Rastogi R.P. Chemical constituents of Tithonia tagitiflora Desf: part IV. Tagitinin C, D & F. Indian J. Chem. 15:1977;208
Baruah N.C., Sharma R.P., Madhusudanan K.P., Thyagarajan G. Sesquiterpene lactones of Tithonia diversifolia. Stereochemistry of the tagitinins and related compounds. J. Org. Chem. 44:1979;1831
Gu J.-Q., Gills J.J., Park E.J., Mata-Greenwood E., Hawthorne M.E., Axelrod F., Chavez P.I., Fong H.H.S., Mehta R.G., Pezzuto J.M., Kinghorn A.D. Sesquiterpenoids from Tithonia diversifolia with potential cancer chemopreventive activity. J. Nat. Prod. 65:2002;532
M. Mukhopadhyay, Natural Extracts using Supercritical Carbon Dioxide, CRC Press, Boca Raton, 2000, p. 339.
L.T. Taylor, Supercritical Fluid Extraction, Wiley-Interscience, New York, 1996, p. 181.
Smith R.M., Burford M.D. Optimization of supercritical fluid extraction of volatile constituents from a model plant matrix. J. Chromatogr. 600:1992;175
Smith R.M., Burford M.D. Supercritical fluid extraction and gas chromatographic determination of the sesquiterpene lactone parthenolide in the medicinal herb feverfew (Tanacetum parthenium). J. Chromatogr. 627:1992;255
Chen S.L., You J., Wang G.J. Supercritical fluid extraction of essential oil from Rhizoma Atractylodes Macrocephalae. Fenxi Ceshi Xuebao. 19:2000;59
Kohler M., Haerdi W., Christen P., Veuthey J.L. Extraction of artemisinin and artemisinic acid from Artemisia annua L. using supercritical carbon dioxide. J. Chromatogr. A. 785:1997;353
Kohler M., Haerdi W., Christen P., Veuthey J.L. Supercritical fluid extraction and chromatography of artemisinin and artemisinic acid. An improved method for the analysis of Artemisia annua samples. Phytochem. Anal. 8:1997;223
Goffin E., Proença da Cunha A., Ziemons E., Tits M., Angenot L., Frederich M. High performance liquid chromatographic quantification of tagitinin C in Tithonia diversifolia (Hemsley) A. Gray. Phytochem. Anal. 14:2003;378
Ziémons E., Goffin E., Lejeune R., Angenot L., Thunus L. FTIR Measurement of Tagitinin C after Solvent Extraction from Tithonia diversifolia. Talanta. 62:2004;383
ICH, Guidance for industry, Q2B Validation of Analytical Procedures: Methodology, Federal Register 62 (1996) 27463.
Drake B., Smith R. Measurement of static dielectric constants of supercritical fluid solvents and cosolvents. J. Supercrit. Fluids. 3:1990;162
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