Improved IL-2 detection for determination of helper T lymphocyte precursor frequency in limiting dilution assay

[en] In the context of allogeneic bone marrow transplantation, an accurate estimate of the risk of developing graft-versus-host disease GVHD. is of major interest. The pre-transplant frequency of donor’s helper T-lymphocyte precursors HTLp. directed against host’s antigens may be helpful in predicting this risk. This technique relies on an indirect measurement of interleukin-2 IL-2. secreted by the HTLp, as assessed by the proliferation of an IL-2 dependent cell line. Many authors use the murine CTLL-2 cell line in this assay, but these cells do not respond to the presence of minute amounts of IL-2 in the culture medium, and thus do not discriminate between the absence or the presence of very low levels of IL-2. We therefore decided to compare CTLL-2 with another IL-2 dependent cell line, the murine A9.12 cell line. A comparison was made using serial dilutions of recombinant human IL-2, limiting dilutions of baby hamster kidney BHK. cells transfected with human IL-2 gene and in the context of clinical tests performed for the detection of pre-transplant HTLp. Both the sensitivity and reliability of the tests were better using A9.12. We conclude that the A9.12 cell line might be a more suitable tool for pre-transplant HTLp determinations before allogeneic bone marrow transplantation or whenever low IL-2 levels are to be measured.

Research Center/Unit :

UCL, Immunohématologie

Disciplines :

Biotechnology

Author, co-author :

Winandy, Marie ; Université Catholique de Louvain - UCL > Immunohématologie

Lewalle, Philippe

Deneys, Véronique

Ferant, Augustin

De Bruyère, Marc

Language :

English

Title :

Improved IL-2 detection for determination of helper T lymphocyte precursor frequency in limiting dilution assay

Publication date :

1998

Journal title :

Journal of Immunological Methods

ISSN :

0022-1759

eISSN :

1872-7905

Publisher :

Elsevier Science

Volume :

215

Pages :

81-94

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique

Available on ORBi :

since 24 July 2013

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