Pretransplant helper T-lymphocyte determination in bone marrow donors: acute graft-versus-host disease prediction and relation with long-term survival

Winandy, Marie; Lewalle, Philippe; Deneys, Véronique; Ferrant, Augustin; De Bruyère, Marc

doi:10.1111/j.1365-2141.1999.01282.x

Article (Scientific journals)

Pretransplant helper T-lymphocyte determination in bone marrow donors: acute graft-versus-host disease prediction and relation with long-term survival

Winandy, Marie; Lewalle, Philippe; Deneys, Véronique et al.

1999 • In British Journal of Haematology, 105, p. 288-294

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/153749

DOI
10.1111/j.1365-2141.1999.01282.x

PubMed
10233396

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Keywords :

helper T-lymphoyte frequency; bone marrow transplantation; GVHD

Abstract :

[en] Helper T-lymphocyte precursor (HTLp) frequency from 19 allogeneic bone marrow donors was tested to detect weak antigenic differences with the recipient, and then compared to the outcome. HTLp frequency was estimated in limiting dilution cultures, and HLA-DR and CD 80 expression by stimulating cells was measured by flow cytometry. 12/19 patients experienced acute graft-versus-host disease (aGVHD) grade II–IV. A good correlation was found between high pretransplant HTLp frequency and grade II–IV aGVHD (median: 1/55848 PBMNC for II–IV GVHD versus 1/ 184346 for 0–I GVHD; P¼0·008). Sensitivity was 82%, specificity 63%, negative predictive value 71% and positive predictive value 75%. Long-term survivors also had a lower HTLp median frequency (1/143354) when compared with patients who died as a result of the transplant procedure (1/22100, P < 0·001). No correlation was found between HTLp frequency and HLA-DR or CD80 expression by patient’s cells. We conclude that HTLp frequency estimation can predict, although poorly, acute GVHD risk and long-term survival.

Research Center/Unit :

Université Catholique de Louvain, Immunohaematology, Brussels

Disciplines :

Hematology

Author, co-author :

Winandy, Marie ; Université Catholique de Louvain - UCL > Faculté de Médecine > Immuno-hématologie

Lewalle, Philippe

Deneys, Véronique

Ferrant, Augustin

De Bruyère, Marc

Language :

English

Title :

Pretransplant helper T-lymphocyte determination in bone marrow donors: acute graft-versus-host disease prediction and relation with long-term survival

Publication date :

1999

Journal title :

British Journal of Haematology

ISSN :

0007-1048

eISSN :

1365-2141

Publisher :

Blackwell Publishing, Oxford, United Kingdom

Volume :

105

Pages :

288-294

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique

Available on ORBi :

since 24 July 2013

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Bibliography

Appleton, A.L. & Sviland, S. (1993) Pathogenesis of GVHD: role of herpes viruses. Bone Marrow Transplantation, 11, 349-355.
Appleton, A.L., Sviland. S., l'eiris, J.S.M., Taylor, C.E., ViIkes, J., Green, M.A., Pearson, A.D.J., Proctor, S.J., Hamilton, P.J., Cant, A.J. & Malcolm, A.J. (1995) Role of target organ infection with cytomegalovirus in the pathogenesis of GVHD. Bone Marrow Transplantation, 15, 557-561.
Aschan, J., Ringdén, O., Andström, E., Ljungman, P., Lönnqvist, B. & Remberger, M. (1994) Individualised prophylaxis against GVHD in leukemic marrow transplant recipients. Bone Marrow Transplantation, 14, 79-87.
Barnett. V. & Lewit. T. (1984) Outliers in Statistical Data. 2nd edn. \Viley. New York.
Boström, L., Ringdén, 0. & Forsgren, M. (1992) Strong donor mononuclear cell reactivity for herpes simplex virus antigen in immune donor/recipient pair is associated with GVHD. Transplantation Proceedings. 24, 376-677.
Bunjes, D., Theobald, M., Nierle, T., Arnold, R. & Heimpcl, H. (1995) Presence of host-specific IL-2-secreting T helper cell precursors correlates closely with active primary and secondary chronic GVHD. Bone Marrow Transplantation, 15, 727-732.
Ferrara. J.L.M. & Deeg. H.J. (1991) Mechanisms of disease: GVHD. New England Journal of Medicine, 324, 667-674.
Gribben, J.G., Guinan, E.C., Boussiotis, V.A., Ke, X.Y., Linsley, L., Sieff, C., Gray, G.S., Freeman. G.J. & Nadler, L.M. (1996) Complete blockade of B7 family mediated costimulation is necessary to induce human alloantigen-specific anergy: a method to ameliorate graft-versus-host disease and extend the donor pool. Blood. 87, 4887-4893.
Hansen, J.A., Petersdorf. E.W., Choo, S.Y., Martin, P.J. & Anasetti, C. (1995) Marrow transplantation from HLA partially matched relatives and unrelated donors. Bone Marron' Transplantation, 15, (Suppl. 2), s128-s129.
Hornick, P., Brookes, P., Mason, P., Taylor, K.M., Yocoub, M.Y., Batchelor, J.R., Rose, M.L. & Lechler, R.I. (1996) Limiting dilution analysis: increasing the sensitivity and specificity of alloreactive T helper cell assay by abrogating 'unwanted' 1L-2 production. Human Immunology, 47, 130.
Kaminski, E., Hows, J., Man, S., Brookes, P., Hughes, T., Avakian. O., Goldman. J.M. & Batchelor, J.R. (1989) Prediction of GVHD by frequency analysis of cytotoxic T cells after unrelated donor bone marrow transplantation. Transplantation, 48, 608-613.
Kalhs, P. (1993) GVHD: risk factors. Bone Marrow Transplantation, 12, (Suppl. 4). s48-s50.
Kaplan. E.L. & Meier. P. (1958) Nonparametric estimations from incomplete observations. Journal of the American Statistical Association. 53. 457-481.
Kecver-Taylor. C.A., Passweg, J., Kawanishi, Y., Casper. J., Flomenberg, N. & Baxter-Löwe, L.A. (1997) Association of donor-derived host-reactive cytolytic and helper T cells with outcome following alternative donor T-cell-depleted bone marrow transplantation. Bone Marrow Transplantation, 19. 1001-1009.
Kelemen, E., Szebeni, J. & Petranyi, G. (1993) GVHD in bone marrow transplantation: experimental. laboratory and clinical contributions of the last few years. International Archives of Allergy and Immunology. 102. 309-320.
Knulst. A.C., Tibbe, G.J.M., Noort. W.A., Bril-Bazuin. C., Benner. R. & Sarclkoul. H.F.J. (1994) Prevention of lethal graft-versus-host disease in mice by monoclonal antibodies directed against T cells or their subsets. I. Evidence for trie induction of a state of tolerance based on suppression. Bone Marrow Transplantation, 13, 293-301.
Lewalle, P., Hensel, N., Guimares, A., Couriel. D., Jiang, Y.Z., Mavroudis, D. & Barrett, A.J. (1996) Helper and cytotoxic lymphocyte responses to chronic myeloid leukaemia: implications for adoptive immunotherapy with T cells. British Journal of Haematology. 92, 587-594.
Mickelson, E.M., Longton, G., Anasetti, C., Petersdorf, E., Martin, P., Guthrie, LA. & Hansen, J.A. (1996) Evaluation of the mixed lymphocyte culture (MLC) assay as a method for selecting unrelated donors for marrow transplantation. Tissue Antigens, 47, 27-36.
Potolicchio. I., Brookes, P.A., Madrigal, A., Lechler, R.I. & Sorrentino. R. (1996) HLA-DPB1 mismatch at position 69 is associated with high helper T lymphocyte precursor frequencies in unrelated bone marrow transplant pairs. Transplantation, 62, 1347-1352.
Przepiorka, D., Weisdorf, D., Martin, P., Klingemann. H.G., Beatty, P., Hows, J. & Thomas, E.D. (1995) Consensus conference on GVHD grading. Bone Marrow Transplantation, 15, 825-828.
Reiseater. A.V., Thorsby, E. & Brinchmann. J.E. (1996) Analysis of alloreactive T lymphocyte precursor frequencies: influence of interleukin-2 produced by the stimulating cells. Journal of Iinnumological Methods, 189, 65-70.
Schwarer, A., Jiang, Y.Z., Deacock, S., Brookes. P.A., Barrett, A.J., Goldman, J.M., Batchelor, J.R. & Lechler, R.I. (1994) Comparison of helper and cytotoxic antirecipient T cell frequencies in unrelated bone marrow transplantation. Transplantation, 58, 1198-1203.
Schwarer, A.P., Jiang, Y.Z., Brookes. P.A., Barrett, A.J., Batchelor, J.R., Goldman, J.M. & Lechler, R.I. (1993) Frequency of anti-recipient alloreactive helper T-cell precusor in donor blood and GVHD after identical-sibling bone marrow transplantation. Lancet, 341, 203-205.
Segall, M., Moreen, H., Edwins, L., Haake, R., Shu, X. & Kersey, J. (1996) Lack of correlation of MLC reactivity with acute graft-versus-host disease and mortality in unrelated bone marrow transplantation. Human Immunology, 49, 49-55.
Shih. C.C.Y., Truitt, R.L., Abramoff, P. & Bortin, M.M. (1983) Relationship between IL-2 receptors and cyclosporine-induced suppression of T leukemia and T helper cells. Transplantation Proceedings, 15, (Suppl. 1), 2394-2397.
Speiser, D.E., Löliger, C.C., Siren, M.K. & Jeannet, M. (1996) Pretransplant cytotoxic donor T-cell activity specific to patient HLA class I antigens correlating with mortality after unrelated bone marrow transplantation. British Journal of Uaematology. 93, 935-939.
Spencer, A., Brookcs, P.A., Kaminski. E., Hows, J.M., Szydlo, R.M., van Rhee, F., Goldman, J.M. & Batchelor. J.R. (1995a) CTLp frequency analyses in bone marrow transplantation with volunteer unrelated donors: value in donor selection. Transplantation, 59, 1302-1308.
Spencer, A., Szydlo, R.M., Brookes, P.A., Kaminski, E., Rule, S., van Rhee. F., Ward. K.N., Hale, G., Waldmann, H., Hows, J.M., Batchelor, J.R. & Goldman, J.M. (1995b) Bone marrow transplantation for chronic myeloid leukemia with volunteer donors using ex vivo or in vivo T-cell depletion: major prognostic impact of HLA class I identity betiveen donor and recipient. Blood, 86. 3590-3597.
Taswell, C. (1984) Limiting dilution assays for the determination of immunocompetent cell frequencies. III. Validity tests for single-hit Poisson model. Journal of linimmological Methods, 72, 29-40.
Theobald, M., Nierle, X, Bunjes. D., Arnold, R. & Heimpel. H. (1992) Host-specific IL-2-secreting T cell precursors as predictors of acute GVHD in bone marrow transplantation between HLA-identical siblings. New England Journal of Medicine, 327, 1613-1617.
Theobald, M. (1995) Allorecognition and GVHD. Bone Marrow Transplantation, 15, 489-498.
van Bekkum, D.W. (1991) What is GVHD? Bone Marrow Transplantation. 7, (Suppl. 2), s110-s111.
van der Meer. A., Joosten, I., Ruiter, J. & Allebes, W.A. (1997) A single [3H]thymidine-based limiting dilution analysis to determine HTLp and CTLp frequencies for bone marrow donor selection. Bone Marrow Transplantation, 20, 149.
Van Gool, S.W., de Boer, M. & Ceuppens, J.L. (1994) The combination of anti-B7 monoclonal antibody and cyclosporin A induces alloantigen-specific anergy during a primary mixed lymphocyte reaction. Journal of Experimental Medicine, 179, 715-720.
\Vinandy, M., Lewalle, P., Deneys, V, Ferrant, A. & De Bruyère, M. (1998) Improved interIeukin-2 detection for determination of helper T lymphocyte precursor frequency in limiting dilution assay. Journal of Immunological Methods, 215, 81-94.