Article (Scientific journals)
Medium effects on micropropagation and genetic stability of Citrullus lanatus oleaginous type
Gnamien, Yah Gwladys; Zoro Bi, Irié Arsène; Kouadio, Yatty Justin et al.
2013In Agricultural Sciences, 04 (07), p. 32-44
Peer reviewed
 

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Keywords :
AFLP; Genetic Stability; In Vitro; Oleaginous Seeds; Organogenesis
Abstract :
[en] To regenerate adventitious shoots from the cotyledon proximal parts of Citrullus lanatus (Thunb.) Matsum. and Nakai ssp. mucosospermus (Fursa) oleaginous type, different concentrations of MS mineral elements, sucrose, 6-benzylaminopurine (BAP) and agar were tested. Shoot induction proved to depend on the interaction between levels of sucrose, BAP and MS mineral elements in the medium. The medium containing 3/2 strength of MS mineral elements, 35 g/l sucrose and 1 mg/l BAP solidified with 6 g/l agar allowed the production of numerous shoots without a callus phase. After 3 weeks of culture, 76.7% of the cotyledon proximal parts induced shoots with an average of 12.26 shoots per explant and a mean shoot length of 17.13 mm. The induced shoots were directly rooted and thus complete plants ready for acclimatization were obtained using a two steps procedure. Depending on the genotype, the shoot induction from cotyledon proximal parts ranged from 54% to 96%. Rooted plantlets were acclimatized and transferred to field, where they grew well, developed flowers and fruits like seeded plants. The assessment of the genetic stability of the in-vitro-regenerated plantlets by means of an Amplified Fragment Length Polymorphism (AFLP) analysis with the combination of 5 primers revealed no differences between regenerated plantlets and mother plants.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Agriculture & agronomy
Author, co-author :
Gnamien, Yah Gwladys
Zoro Bi, Irié Arsène
Kouadio, Yatty Justin
Brostaux, Yves  ;  Université de Liège - ULiège > Sciences agronomiques > Statistique, Inform. et Mathém. appliquée à la bioingénierie
Baudoin, Jean-Pierre ;  Université de Liège - ULiège > Sciences agronomiques > Phytotechnie tropicale et horticulture
Language :
English
Title :
Medium effects on micropropagation and genetic stability of Citrullus lanatus oleaginous type
Publication date :
2013
Journal title :
Agricultural Sciences
ISSN :
2156-8553
eISSN :
2156-8561
Publisher :
Scientific Research Publishing, Irvine, United States - California
Volume :
04
Issue :
07
Pages :
32-44
Peer reviewed :
Peer reviewed
Available on ORBi :
since 24 July 2013

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