Purification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalis placentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females.

Barbato, Olimpia; Melo de Sousa, Noelita; Barile, Vittoria Lucia; Canali, Claudio; Beckers, Jean-François

doi:10.1186/1746-6148-9-89

Article (Scientific journals)

Purification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalis placentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females.

Barbato, Olimpia; Melo de Sousa, Noelita; Barile, Vittoria Lucia et al.

2013 • In BMC Veterinary Research, 9, p. 89

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https://hdl.handle.net/2268/151524

DOI
10.1186/1746-6148-9-89

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23634647

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Keywords :

Water buffalo; Placenta; Pregnancy-associated glycoprotein; Purification; Radioimmunoassay; Polyclonal; Pregnancy diagnosis; N-terminal amino acid sequence

Abstract :

[en] BACKGROUND: Pregnancy-associated glycoproteins (PAGs) were first described as placental antigens present in the blood serum of the mother soon after implantation. Here, we describe the purification of several pregnancy-associated glycoproteins from water buffalo placenta (wbPAGs). A specific radioimmunoassay (RIA) was developed for early pregnancy diagnosis in buffalo species. RESULTS: Amino-terminal microsequencing of immunoreactive placental proteins allowed the identification of eleven wbPAGs sequences [Swiss-Prot accession numbers: P86369 to P86379]. Three polyclonal antisera (AS#858, AS#859 and AS#860) were raised in rabbits against distinct wbPAG fractions. A new RIA (RIA-860) was developed and used to distinguish between pregnant (n = 33) and non-pregnant (n = 26) water buffalo females. CONCLUSIONS: Our results confirmed the multiplicity of PAG expression in buffalo placenta. In addition, the RIA-860 system was shown to be sensitive, linear, reproducible, accurate and specific in measuring PAG concentrations in buffalo plasma samples from Day 37 of gestation onwards.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Barbato, Olimpia; University of Perugia, Perugia 06126, Italy > Department of Biopathological Veterinary Science, Faculty of Veterinary Medicine

Melo de Sousa, Noelita ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction

Barile, Vittoria Lucia

Canali, Claudio

Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction

Language :

English

Title :

Purification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalis placentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females.

Publication date :

2013

Journal title :

BMC Veterinary Research

eISSN :

1746-6148

Publisher :

BioMed Central, United Kingdom

Volume :

Pages :

Peer reviewed :

Peer Reviewed verified by ORBi

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since 26 June 2013

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