Reference : Evidence for cross-talk between the LH receptor and LH during implantation in mice
Scientific journals : Article
Human health sciences : Endocrinology, metabolism & nutrition
Human health sciences : Reproductive medicine (gynecology, andrology, obstetrics)
http://hdl.handle.net/2268/151506
Evidence for cross-talk between the LH receptor and LH during implantation in mice
English
Gridelet, Virginie mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Embryologie >]
Tsampalas, Marie [> >]
Berndt, Sarah [> >]
Hagelstein, Marie-Thérèse [> >]
CHARLET, Jeanne de Chantal [Centre Hospitalier Universitaire de Liège - CHU > > Endocrinologie clinique >]
Ectors, Fabien mailto [Université de Liège - ULiège > > GIGA-Management : Plate-forme transgénique >]
Hugé, Fabian [> >]
Munaut, Carine mailto [Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
Foidart, Jean-Michel mailto [Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique >]
Geenen, Vincent mailto [Université de Liège - ULiège > > Centre d'immunologie >]
Perrier d'Hauterive, Sophie mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Embryologie >]
2013
Reproduction, Fertility and Development
CSIRO Publishing
25
511-522
Yes (verified by ORBi)
International
1031-3613
East Melbourne
Australia
[en] Embryo ; Implantation ; Luteinizing hormone (LH) ; LH-hCG receptor
[en] The present study investigated the first interaction that occurs between the blastocyst and endometrium during implantation. Given the ethical objections to studying implantation in humans, a mouse model was used to study the dialogue between luteinising hormone (LH) and luteinising hormone receptor (LHCGR). Several studies performed on LHCGR-knockout mice have generated controversy regarding the importance of the dialogue between LH and LHCGR
during implantation. There has been no demonstration of a bioactive LH-like signal produced by the murine blastocyst.
The first aim of the present study was to examine and quantify, using radioimmunoassay, the generation of a bioactive LH signal by the murine blastocyst. We went on to examine and quantify endometrial Lhcgr expression to validate the mouse model. Expression of LHCGR in mouse uteri was demonstrated using immunohistochemistry and western blot analysis. To quantify the expression of Lh in the mouse blastocyst and Lhcgr in the endometrium, reverse transcription–polymerase chain reaction (RT-PCR) and real-time quantitative (q) RT-PCR were performed. The results demonstrate that Lhcgr expression in BALB/c mouse endometrial epithelium is increased at the time of implantation and indicate that LHCGR may contribute to the implantation process. In support of this hypothesis, we identified a bioactive LH signal at the time of murine blastocyst implantation.
Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Union Européenne = European Union - UE = EU (FP6)
EMBIC
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/151506
10.1071/RD11241

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