Article (Scientific journals)
Evidence for cross-talk between the LH receptor and LH during implantation in mice
Gridelet, Virginie; Tsampalas, Marie; Berndt, Sarah et al.
2013In Reproduction, Fertility and Development, 25, p. 511-522
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Keywords :
Embryo; Implantation; Luteinizing hormone (LH); LH-hCG receptor
Abstract :
[en] The present study investigated the first interaction that occurs between the blastocyst and endometrium during implantation. Given the ethical objections to studying implantation in humans, a mouse model was used to study the dialogue between luteinising hormone (LH) and luteinising hormone receptor (LHCGR). Several studies performed on LHCGR-knockout mice have generated controversy regarding the importance of the dialogue between LH and LHCGR during implantation. There has been no demonstration of a bioactive LH-like signal produced by the murine blastocyst. The first aim of the present study was to examine and quantify, using radioimmunoassay, the generation of a bioactive LH signal by the murine blastocyst. We went on to examine and quantify endometrial Lhcgr expression to validate the mouse model. Expression of LHCGR in mouse uteri was demonstrated using immunohistochemistry and western blot analysis. To quantify the expression of Lh in the mouse blastocyst and Lhcgr in the endometrium, reverse transcription–polymerase chain reaction (RT-PCR) and real-time quantitative (q) RT-PCR were performed. The results demonstrate that Lhcgr expression in BALB/c mouse endometrial epithelium is increased at the time of implantation and indicate that LHCGR may contribute to the implantation process. In support of this hypothesis, we identified a bioactive LH signal at the time of murine blastocyst implantation.
Disciplines :
Endocrinology, metabolism & nutrition
Reproductive medicine (gynecology, andrology, obstetrics)
Author, co-author :
Gridelet, Virginie ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Embryologie
Tsampalas, Marie
Berndt, Sarah
Hagelstein, Marie-Thérèse
Charlet-Renard, Jeanne de Chantal ;  Centre Hospitalier Universitaire de Liège - CHU > Endocrinologie clinique
Ectors, Fabien  ;  Université de Liège - ULiège > GIGA-Management : Plate-forme transgénique
Hugé, Fabian
Munaut, Carine  ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Foidart, Jean-Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Geenen, Vincent ;  Université de Liège - ULiège > Centre d'immunologie
Perrier d'Hauterive, Sophie ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Embryologie
Language :
English
Title :
Evidence for cross-talk between the LH receptor and LH during implantation in mice
Publication date :
2013
Journal title :
Reproduction, Fertility and Development
ISSN :
1031-3613
eISSN :
1448-5990
Publisher :
CSIRO Publishing, East Melbourne, Australia
Volume :
25
Pages :
511-522
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
EMBIC
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
UE - Union Européenne [BE]
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