Article (Scientific journals)
From dormant to germinating spores of Streptomyces coelicolor A3(2): new perspectives from the crp null mutant.
Piette, André; Derouaux, Adeline; Gerkens, Pascal et al.
2005In Journal of Proteome Research, 4 (5), p. 1699-708
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Keywords :
Alleles; Amino Acid Sequence; Bacterial Proteins/chemistry; Cyclic AMP Receptor Protein/genetics; Electrophoresis, Gel, Two-Dimensional; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genetic Techniques; Mass Spectrometry; Microscopy, Electron; Molecular Sequence Data; Mutation; Open Reading Frames; Phenotype; Proteomics/methods; Spores, Bacterial; Streptomyces coelicolor/metabolism; Trypsin/pharmacology
Abstract :
[en] The complete understanding of the morphological differentiation of streptomycetes is an ambitious challenge as diverse sensors and pathways sensitive to various environmental stimuli control the process. Germination occupies a particular position in the life cycle as the good achievement of the process depends on events occurring both during the preceding sporulation and during germination per se. The cyclic AMP receptor protein (crp) null mutant of Streptomyces coelicolor, affected in both sporulation and germination, was therefore presented as a privileged candidate to highlight new proteins involved in the shift from dormant to germinating spores. Our multidisciplinary approach-combining in vivo data, the analysis of spores morphological properties, and a proteome study-has shown that Crp is a central regulatory protein of the life cycle in S. coelicolor; and has identified spores proteins with statistically significant increased or decreased expression that should be listed as priority targets for further investigations on proteins that trigger both ends of the life cycle.
Disciplines :
Microbiology
Author, co-author :
Piette, André ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Derouaux, Adeline ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Gerkens, Pascal
Noens, Elke E E
Mazzucchelli, Gabriel  ;  Université de Liège - ULiège > Centre interfac. d'analyse des résidus en traces (CART)
Vion, Sebastien
Koerten, Henk K
Titgemeyer, Fritz
De Pauw, Edwin  ;  Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Leprince, Pierre ;  Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
van Wezel, Gilles P
Galleni, Moreno ;  Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Rigali, Sébastien  ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
More authors (3 more) Less
Language :
English
Title :
From dormant to germinating spores of Streptomyces coelicolor A3(2): new perspectives from the crp null mutant.
Publication date :
September 2005
Journal title :
Journal of Proteome Research
ISSN :
1535-3893
eISSN :
1535-3907
Publisher :
American Chemical Society, Washington, United States - District of Columbia
Volume :
4
Issue :
5
Pages :
1699-708
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 June 2009

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