Reference : Ultraviolet Laser Induced Hydrogen Transfer Reaction: Study of the First Step of MALD...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/145680
Ultraviolet Laser Induced Hydrogen Transfer Reaction: Study of the First Step of MALDI In-Source Decay Mass Spectrometry
English
Asakawa, Daiki []
Calligaris, David [Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Smargiasso, Nicolas mailto [Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
De Pauw, Edwin mailto [Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
29-Jan-2013
Journal of Physical Chemistry B
American Chemical Society
117
8
2321-2327
Yes (verified by ORBi)
International
1520-6106
1520-5207
Washington
DC
[en] Mass Spectrometry ; MALDI ; In source decay
[en] The early mechanisms of matrix-assisted laser desorption/ionization in-source decay (MALDI-ISD) are described herein. MALDI-ISD is initiated by the hydrogen transfer from excited matrix molecules to the carbonyl oxygen of the peptide backbone, which is followed by a radical-induced cleavage, producing the c′/z• fragment pair. As expected, the use of 2,5-DHB or 1,5-DAN was efficient to induce MALDI-ISD, and the strongest intensity of MALDI-ISD fragments was observed when laser shots were performed on matrix crystals. In contrast, the hydrogen radical transfer reaction was suppressed by using ionic liquid and amorphous structure of 2,5-DHB and 1,5-DAN mixture as a matrix. Our results suggest that the hydrogen transfer occurs on the matrix crystal during the dissipation of the laser energy and before desorption, following ISD fragments formed in the MALDI plume.
Giga-Systems Biology and Chemical Biology
Japan Society for the Promotion of Science for Young Scientists (23-10272) ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; University of Liege ARC
Researchers ; Professionals
http://hdl.handle.net/2268/145680
10.1021/jp311464k
http://pubs.acs.org/journal/jpcbfk

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