[en] Sorting of soft-bottom macrobenthos entangled with Posidonia oceanica fibers is time-consuming and tedious because of the weak of colour contrast . This paper describes a staining-destaining technique that produces good contrast between soft-bottom macrobenthos and Posidonia oceanica fibers. The method has been tested on Corsican samples in oligotrophic areas characterized by small-size soft-bottom macrofauna. Our technique saves sorting time (-24%) and is simple to implement.
Disciplines :
Aquatic sciences & oceanology
Author, co-author :
Donnay, Annick ; Université de Liège - ULiège et STARESO > BEE > Océanologie
Pelaprat, C; STARESO sas
Lejeune, P; STARESO sas
Gobert, Sylvie ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Océanologie
Language :
English
Title :
An adapted staining-destaining method to sort soft-bottom macrobenthos mixed with Posidonia oceanica fibers
Publication date :
2013
Journal title :
Mediterranean Marine Science
ISSN :
1108-393X
eISSN :
1791-6763
Publisher :
National Centre for Marine Research, Athens, Greece
Volume :
14
Issue :
1
Pages :
92-94
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Race ARC-05/10-333; 2.4502.08.F
Funders :
FWB - Fédération Wallonie-Bruxelles [BE] F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] Agence de l'eau Rhône Méditerranée Corse [FR] Collectivity of Corsica [FR]
Dauvin, J.-C., Ruellet, T., Desroy, N., Janson, A.-L., 2007. The ecological quality status of the Bay of Seine and the Seine estuary: Use of biotic indices. Marine Pollution Bulletin, 55 (1-6), 241-257.
ISO, 2005. Water quality: guidelines for quantitative sampling and sample processing of marine soft-bottom macrofauna. ISO/FDIS 16665:2005(E), 30 pp.
Labrune, C., Grémare, A., Guizien, K., Amouroux, J.-M., 2007. Long-term comparison of soft-bottom macrobenthos in the Bay of Banyuls-sur-Mer (north-western Mediterranean Sea): a reappraisal. Journal of Sea Research, 58 (2), 125-143.
Mangialajo, L., Ruggieri, N., Asnaghi, V., Chiantore, M., Povero, P. et al., 2007. Ecological status in the Ligurian Sea: the effect of coastline urbanisation and the importance of proper reference sites. Marine Pollution Bulletin, 55 (1-6), 30-41.
Massé, H.L., 2000. Long-term changes in sand-bottom macrofauna along the coast of Provence (northwest Mediterranean Sea). Oceanologica Acta, 23 (2), 229-242.
Mateo, M.A., Romero, J., 1997. Detritus dynamics in the seagrass Posidonia oceanica: Elements for an ecosystem carbon and nutrient budget. Marine Ecology Progress Series, 151, 43-53.
Thiel, H., 1966. Quantitative Untersuchungen über die Meiofauna des Tiefseebodens. Veröffentli Veröffentlichungen des Instituts für Meeresforschung in Bremerhaven, 2, 131-148.
Williams, G.E., 1974. New technique to facilitate hand-picking macrobenthos. Transactions of the American Microscopical Society, 93 (2), 220-226