Article (Scientific journals)
Proteomic kinetic analysis of blister fluid and serum in a patient with drug-induced toxic epidermal necrolysis. A comparison with skin immunohistochemistry.
Paquet, Philippe; Meuwis, Marie-Alice; Mazzucchelli, Gabriel et al.
2012In Current Drug Safety, 7 (5), p. 339-51
Peer Reviewed verified by ORBi
 

Files


Full Text
PROTEOMIC KINETIC.pdf
Publisher postprint (858.12 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] Drug-induced toxic epidermal necrolysis (TEN) is a rare but potentially lethal bullous disease whose complex pathomechanisms remain uncertain. The aim of the study was an exploratory attempt to assess TEN pathobiology using a combination of immunohistochemistry and proteomics. Skin blister fluid (BF) and serum were collected in a patient in the early TEN stage at day (D) +4 of evolution and three days later (D +7). Intravenous cyclosporine A (CsA) treatment was initiated since D +4. Immunohistochemistry was performed on skin blister biopsies. In addition, proteomic analyses compared the BF protein content before and at the issue of the 3-day CsA treatment. Proteins were selected according to their prominent differential abundance in BF between D+4 and D+7, when influenced by lesional skin cells, but not in serum. Among 300 proteins, four were considered. Glutathione transferase pi was related to oxidative stress in TEN epidermis. The monocyte differentiation antigen CD14 and myeloperoxidase indicated macrophage activation. The proinflammatory S100-A8 protein probably originated from activated keratinocytes and/or macrophages. These proteomic findings were in line with immunohistochemistry and supported the prominent involvement of keratinocytes and macrophages in TEN pathomechanism. As opposed to CD14, other proteins were mainly present in BF at D+7, confirming that CsA expressed little effect, if any, on the activity of keratinocytes and macrophages in the present TEN patient. Of note, the present exploratory study using proteomic analyses in a single TEN case supports a pathogenic hypothesis without establishing any firm conclusion.
Disciplines :
Dermatology
Author, co-author :
Paquet, Philippe
Meuwis, Marie-Alice  ;  Centre Hospitalier Universitaire de Liège - CHU > Gastro-Entérologie-Hépatologie
Mazzucchelli, Gabriel  ;  Université de Liège - ULiège > Center for Analytical Research and Technology (CART)
Delvenne, Philippe ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
PIERARD, Gérald ;  Centre Hospitalier Universitaire de Liège - CHU > Dermatopathologie
Language :
English
Title :
Proteomic kinetic analysis of blister fluid and serum in a patient with drug-induced toxic epidermal necrolysis. A comparison with skin immunohistochemistry.
Publication date :
2012
Journal title :
Current Drug Safety
ISSN :
1574-8863
eISSN :
2212-3911
Publisher :
Bentham Science Publishers, United Arab Emirates
Volume :
7
Issue :
5
Pages :
339-51
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 01 March 2013

Statistics


Number of views
75 (8 by ULiège)
Number of downloads
1 (1 by ULiège)

Scopus citations®
 
8
Scopus citations®
without self-citations
7
OpenCitations
 
7

Bibliography


Similar publications



Contact ORBi