Reference : Cost-effective media for the rapid and high resolution of small DNA fragments using p...
Scientific journals : Article
Life sciences : Environmental sciences & ecology
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/140327
Cost-effective media for the rapid and high resolution of small DNA fragments using polyacrylamide-based electrophoresis
English
Negro, S. S. [Molecular Ecology Laboratory, School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, New Zealand]
Cloots, Rudi [Université de Liège - ULiège > Département de chimie (sciences) > LCIS - GreenMAT >]
Gemmell, N. J. [Molecular Ecology Laboratory, School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, New Zealand]
2006
Molecular Ecology Notes
Blackwell Publishing
6
3
609-612
Yes (verified by ORBi)
International
1471-8278
1471-8286
Oxford
United Kingdom
[en] Alkali salt ; Gel-electrophoresis ; Microsatellite
[en] Current Tris-based solutions for DNA electrophoresis produce a positive feedback loop between current and temperature at high voltage, resulting in long running times for the separation of even small DNA fragments. We optimized the separation of small DNA fragments (90-300 bp) in polyacrylamide-based electrophoresis at high voltages (200volts/cm) by substituting Tris with low concentration alkali salts (e.g. 1 mm LiCl and CsCl). These media reduced the heat produced during electrophoresis, enhanced the DNA fragment resolution, and allowed gels to be run at higher voltages, reducing gel running times by 25%. In addition, the elimination of Tris and EDTA from the buffer reduced material costs approximately 10-fold. © 2006 The Authors.
http://hdl.handle.net/2268/140327
10.1111/j.1471-8286.2006.01290.x

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