Reference : Optimization of Production and Preliminary Characterization of New Exopolysaccharides...
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Multidisciplinary, general & others
Optimization of Production and Preliminary Characterization of New Exopolysaccharides from Gluconacetobacter hansenii LMG1524
Valepyn, Emmanuel mailto [Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle >]
Berezina, Nathalie mailto [MateriaNova, rue des foudriers, 1, 7822 Ghislenghien > > > >]
Paquot, Michel mailto [Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle >]
Advances in Microbiology
Scientific Research Publishing
Irvine (CA)
[en] Gluconacetobacter hansenii ; extracellular polysaccharides ; culture conditions ; medium composition ; orthogonal design
[en] The influence of different carbon and nitrogen sources, of ethanol concentration, the optimal pH, temperature and me-dium composition were evaluated on extracellular polysaccharides (EPS) synthesis and bacterial growth of Gluconace-tobacter hansenii LMG1524, and preliminary characterization of EPS was investigated. The highest EPS yields were obtained using glycerol and ammonium sulphate as carbon and nitrogen sources, respectively. The increase of ethanol concentration in the medium did not influence the EPS synthesis but reduced the bacterial growth. The optimum tem-perature and pH for polysaccharides production were respectively 25 °C and 5; whereas for cell growth were respec-tively 30 °C and 4. The optimal culture medium composition was determined as follows : 10 g/L sucrose, 0.892 g/L (NH4)2SO4, 0.34 g/L NaNO3, 3 mL acetic acid, 1.5 g/L KH2PO4, 1.5 g/L K2HPO4 and 0.6 g/L MgSO4. The polysaccha-rides produced were of 14 and 10 polymerization degrees (DP) and constituted mainly of glucose, galactose and man-nose, in relative percent of 36.36, 33.94 and 22.42, respectively.
Fonds Européen de Développement Régional - FEDER
Researchers ; Professionals ; Students

File(s) associated to this reference

Fulltext file(s):

Restricted access
AiM20120400011_29609564.pdfPublisher postprint217.06 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.