Article (Scientific journals)
Optimization of Production and Preliminary Characterization of New Exopolysaccharides from Gluconacetobacter hansenii LMG1524
Valepyn, Emmanuel; Berezina, Nathalie; Paquot, Michel
2012In Advances in Microbiology, 2 (4), p. 488-496
Peer reviewed
 

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Keywords :
Gluconacetobacter hansenii; extracellular polysaccharides; culture conditions; medium composition; orthogonal design
Abstract :
[en] The influence of different carbon and nitrogen sources, of ethanol concentration, the optimal pH, temperature and me-dium composition were evaluated on extracellular polysaccharides (EPS) synthesis and bacterial growth of Gluconace-tobacter hansenii LMG1524, and preliminary characterization of EPS was investigated. The highest EPS yields were obtained using glycerol and ammonium sulphate as carbon and nitrogen sources, respectively. The increase of ethanol concentration in the medium did not influence the EPS synthesis but reduced the bacterial growth. The optimum tem-perature and pH for polysaccharides production were respectively 25 °C and 5; whereas for cell growth were respec-tively 30 °C and 4. The optimal culture medium composition was determined as follows : 10 g/L sucrose, 0.892 g/L (NH4)2SO4, 0.34 g/L NaNO3, 3 mL acetic acid, 1.5 g/L KH2PO4, 1.5 g/L K2HPO4 and 0.6 g/L MgSO4. The polysaccha-rides produced were of 14 and 10 polymerization degrees (DP) and constituted mainly of glucose, galactose and man-nose, in relative percent of 36.36, 33.94 and 22.42, respectively.
Disciplines :
Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others
Author, co-author :
Valepyn, Emmanuel ;  Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Berezina, Nathalie;  MateriaNova, rue des foudriers, 1, 7822 Ghislenghien
Paquot, Michel ;  Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Language :
English
Title :
Optimization of Production and Preliminary Characterization of New Exopolysaccharides from Gluconacetobacter hansenii LMG1524
Publication date :
28 December 2012
Journal title :
Advances in Microbiology
ISSN :
2165-3402
eISSN :
2165-3410
Publisher :
Scientific Research Publishing, Irvine (CA), United States
Volume :
2
Issue :
4
Pages :
488-496
Peer reviewed :
Peer reviewed
Name of the research project :
BIOTISS FUSAGx
Funders :
FEDER - Fonds Européen de Développement Régional [BE]
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since 23 January 2013

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